BMRB Entry 15694

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15694

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Title:
Apple Domains 1 and 2 from Toxoplasma gondii microneme protein 4
Deposition date:
2008-03-26
Original release date:
2008-07-08
Authors:
Marchant, Jan; Sawmynaden, Kovilen; Saouros, Savvas; Simpson, Peter; Matthews, Steve
Citation:

Citation: Marchant, Jan; Sawmynaden, Kovilen; Saouros, Savvas; Simpson, Peter; Matthews, Stephen. "Complete resonance assignment of the first and second apple domains of MIC4 from Toxoplasma gondii, using a new NMRView-based assignment aid"  Biomol. NMR Assignments 2, 119-121 (2008).
PubMed: 19636884

Assembly members:

Assembly members:
MIC4_Ap12, polymer, 174 residues, 18711.6 Da.

Natural source:

Natural source:   Common Name: Toxoplasma gondii   Taxonomy ID: 5811   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Toxoplasma gondii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-32 Xa/LIC

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MIC4_Ap12: SSEPAKLDLSCVHSDNKGSR APTIGEPVPDVSLEQCAAQC KAVDGCTHFTYNDDSKMCHV KEGKPDLYDLTGGKTASRSC DRSCFEQHVSYEGAPDVMTA MVTSQSADCQAACAADPSCE IFTYNEHDQKCTFKGRGFSA FKERGVLGVTSGPKQFCDEG GKLTQEEMEDQISG

Data sets:
Data typeCount
13C chemical shifts649
15N chemical shifts168
1H chemical shifts989

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1protein1

Entities:

Entity 1, protein 174 residues - 18711.6 Da.

1   SERSERGLUPROALALYSLEUASPLEUSER
2   CYSVALHISSERASPASNLYSGLYSERARG
3   ALAPROTHRILEGLYGLUPROVALPROASP
4   VALSERLEUGLUGLNCYSALAALAGLNCYS
5   LYSALAVALASPGLYCYSTHRHISPHETHR
6   TYRASNASPASPSERLYSMETCYSHISVAL
7   LYSGLUGLYLYSPROASPLEUTYRASPLEU
8   THRGLYGLYLYSTHRALASERARGSERCYS
9   ASPARGSERCYSPHEGLUGLNHISVALSER
10   TYRGLUGLYALAPROASPVALMETTHRALA
11   METVALTHRSERGLNSERALAASPCYSGLN
12   ALAALACYSALAALAASPPROSERCYSGLU
13   ILEPHETHRTYRASNGLUHISASPGLNLYS
14   CYSTHRPHELYSGLYARGGLYPHESERALA
15   PHELYSGLUARGGLYVALLEUGLYVALTHR
16   SERGLYPROLYSGLNPHECYSASPGLUGLY
17   GLYLYSLEUTHRGLNGLUGLUMETGLUASP
18   GLNILESERGLY

Related Database Links:

BMRB 18039
PDB 4A5V

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SPARKY: Backbone or all simulated peaks