BMRB Entry 15692

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15692

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Title:
Backbone assignment of MIC1-CT from Toxoplasma gondii in complex with EGF3 from TgMIC6
Deposition date:
2008-03-25
Original release date:
2008-10-15
Authors:
Saouros, Savvas; Sawmynaden, Kovilen; Simpson, Peter; Matthews, Stephen
Citation:

Citation: Saouros, Savvas; Sawmynaden, Kovilen; Marchant, Jan; Simpson, Peter; Matthews, Stephen. "Complete resonance assignment of the galectin-like domain of MIC1 from Toxoplasma gondii in complex with the second EGF domain from MIC6 and the backbone assignment in complex with the third EGF domain"  Biomol. NMR Assignments 2, 175-177 (2008).
PubMed: 19636898

Assembly members:

Assembly members:
TgMIC1-CT, polymer, 138 residues, Formula weight is not available
TgMIC6-EGF3, polymer, 69 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Toxoplasma gondii   Taxonomy ID: 5811   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Toxoplasma gondii

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 21b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TgMIC1-CT: MKTEIHGDSTKATLEEGQQL TLTFISTKLDVAVGSCHSLV ANFLDGFLKFQTGSNSAFDV VEVEEPAGPAVLTIGLGHKG RLAVVLDYTRLNAALGSAAY VVEDSGCSSSEEVSFQGVGS GATLVVTTLGESPTAVSA
TgMIC6-EGF3: IVRQESGCEENGCGPPDAVQ SCRRLTGTAGRLCVCKENFI ATIDASAHITCKRVPPHYRK PPFEFGKGG

Data sets:
Data typeCount
13C chemical shifts355
15N chemical shifts115
1H chemical shifts396

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TgMIC1-CT1
2TgMIC6-EGF32

Entities:

Entity 1, TgMIC1-CT 138 residues - Formula weight is not available

1   METLYSTHRGLUILEHISGLYASPSERTHR
2   LYSALATHRLEUGLUGLUGLYGLNGLNLEU
3   THRLEUTHRPHEILESERTHRLYSLEUASP
4   VALALAVALGLYSERCYSHISSERLEUVAL
5   ALAASNPHELEUASPGLYPHELEULYSPHE
6   GLNTHRGLYSERASNSERALAPHEASPVAL
7   VALGLUVALGLUGLUPROALAGLYPROALA
8   VALLEUTHRILEGLYLEUGLYHISLYSGLY
9   ARGLEUALAVALVALLEUASPTYRTHRARG
10   LEUASNALAALALEUGLYSERALAALATYR
11   VALVALGLUASPSERGLYCYSSERSERSER
12   GLUGLUVALSERPHEGLNGLYVALGLYSER
13   GLYALATHRLEUVALVALTHRTHRLEUGLY
14   GLUSERPROTHRALAVALSERALA

Entity 2, TgMIC6-EGF3 69 residues - Formula weight is not available

1   ILEVALARGGLNGLUSERGLYCYSGLUGLU
2   ASNGLYCYSGLYPROPROASPALAVALGLN
3   SERCYSARGARGLEUTHRGLYTHRALAGLY
4   ARGLEUCYSVALCYSLYSGLUASNPHEILE
5   ALATHRILEASPALASERALAHISILETHR
6   CYSLYSARGVALPROPROHISTYRARGLYS
7   PROPROPHEGLUPHEGLYLYSGLYGLY

Samples:

sample_1: TgMIC1-CT, [U-100% 13C; U-100% 15N], 1-1.3 mM; TgMIC6-EGF3, [U-100% 13C; U-100% 15N], 1-1.3 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.9; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker DRX 500 MHz

Related Database Links:

PDB
EMBL CAA96466
GB EPR59679 EPT28103 ESS33997 KFG30856 KFG41657 AAD28185 EPR63801 EPT24678 ESS28844 KFG37976
REF XP_002368531 XP_002370636
SP O00834 Q9XYH7
TPE CEL76086
AlphaFold O00834 Q9XYH7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks