BMRB Entry 15691

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PDB ID: 2k29
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15691
MolProbity Validation Chart

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Title:
Structure of the DBD domain of E. coli antitoxin RelB
Deposition date:
2008-03-23
Original release date:
2008-06-27
Authors:
Li, Guang-Yao; Zhang, Yonglong; Inouye, Masayori; Ikura, Mitsuhiko
Citation:

Citation: Li, Guang-Yao; Zhang, Yonglong; Inouye, Masayori; Ikura, Mitsuhiko. "Structural mechanism of transcriptional autorepression of the Escherichia coli RelB/RelE antitoxin/toxin module"  J. Mol. Biol. 380, 107-119 (2008).
PubMed: 18501926

Assembly members:

Assembly members:
RelB, polymer, 53 residues, 5993.9 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RelB: GSHMGSINLRIDDELKARSY AALEKMGVTPSEALRLMLEY IADNERLPFKQTL

Data sets:
Data typeCount
15N chemical shifts52
1H chemical shifts362
residual dipolar couplings42

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_21

Entities:

Entity 1, entity_1 53 residues - 5993.9 Da.

Residues 1-3 represent a non-native affinity tag. This is a DNA binding domain of a antitoxin protein.

1   GLYSERHISMETGLYSERILEASNLEUARG
2   ILEASPASPGLULEULYSALAARGSERTYR
3   ALAALALEUGLULYSMETGLYVALTHRPRO
4   SERGLUALALEUARGLEUMETLEUGLUTYR
5   ILEALAASPASNGLUARGLEUPROPHELYS
6   GLNTHRLEU

Related Database Links:

PDB 2K29 4FXE
DBJ BAA15263 BAI25453 BAJ43364 BAN94923
EMBL CAA26250 CAQ98463 CBG34538 CBK86917 CCF87937
GB AAC74637 AAN43138 AAP17029 AAS76441 ABF03723
REF NP_416082 NP_707431 WP_000534857 WP_000534858 WP_001408098
SP P0C079 P0C080
AlphaFold P0C079 P0C080

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SPARKY: Backbone or all simulated peaks