BMRB Entry 15682

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Epidermal Growth Factor-like 2 domain from Microneme Protein 6 (Toxoplasma gondii)
Published: 2008-10-03

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15682

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Epidermal Growth Factor-like 2 domain from Microneme Protein 6 (Toxoplasma gondii)

Deposition date:

2008-03-14

Original release date:

2008-10-03

Authors:

Sawmynaden, Kovilen; Saouros, Savvas; Marchant, Jan; Simpson, Peter; Matthews, Stephen

Citation:

Citation: Sawmynaden, Kovilen; Saouros, Savvas; Marchant, Jan; Simpson, Peter; Matthews, Stephen. "Complete NMR assignments for the second EGF domain of MIC6 from Toxoplasma gondii and re-assignment in complex with the galectin-like domain of MIC1" Biomol. NMR Assignments 2, 187-189 (2008).
PubMed: 19636901

Assembly members:

Assembly members:
Epidermal_Growth_Factor-like_domain_2, polymer, 61 residues, 6362 Da.

Natural source:

Natural source: Common Name: Toxoplasma gondii Taxonomy ID: 5811 Superkingdom: Eukaryota Kingdom: not available Genus/species: Toxoplasma gondii

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-32 Xa/LIC

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Epidermal_Growth_Factor-like_domain_2: MANFVQLSETPAACSSNPCG PEAAGTCKETNSGYICRCNQ GYRISLDGTGNVTCIVRQES G

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	218
15N chemical shifts	54
1H chemical shifts	332

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Epidermal Growth Factor-like domain 2	1

Entities:

Entity 1, Epidermal Growth Factor-like domain 2 61 residues - 6362 Da.

1

MET

ALA

ASN

PHE

VAL

GLN

LEU

SER

GLU

THR

2

PRO

ALA

CYS

SER

ASN

PRO

CYS

GLY

3

PRO

GLU

ALA

GLY

THR

CYS

LYS

GLU

THR

4

ASN

SER

GLY

TYR

ILE

CYS

ARG

CYS

ASN

GLN

5

GLY

TYR

ARG

ILE

SER

LEU

ASP

GLY

THR

GLY

6

ASN

VAL

THR

CYS

ILE

VAL

ARG

GLN

GLU

SER

7

GLY

Samples:

sample_1: Epidermal Growth Factor-like domain 2, [U-98% 13C; U-98% 15N], 0.5 mM; potassium phosphate 25 mM; sodium chloride 50 mM

sample_conditions_1: pH: 6.8; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HC(CO)NH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - Peak picking, Viewing NMR spectra

NMR spectrometers:

Bruker DRX 500 MHz

BMRB	15685
PDB	2K2S 2K2T