BMRB Entry 15677

Name: Solubilization of transmembrane proteins in water: structural studies of a water-soluble analogue of the potassium channel KcsA
Published: 2008-11-10

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PDB ID: 2k1e
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15677
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solubilization of transmembrane proteins in water: structural studies of a water-soluble analogue of the potassium channel KcsA

Deposition date:

2008-02-29

Original release date:

2008-11-10

Authors:

Ma, Dejian; Xu, Yan; Tillman, Tommy; Tang, Pei; Meirovitch, Eva; Eckenhoff, Roderic; Carnini, Anna

Citation:

Citation: Ma, Dejian; Tillman, Tommy; Tang, Pei; Meirovitch, Eva; Eckenhoff, Roderic; Carnini, Anna; Xu, Yan. "NMR studies of a channel protein without membranes: structure and dynamics of water-solubilized KcsA" Proc. Natl. Acad. Sci. U. S. A. 105, 16537-16542 (2008).
PubMed: 18948596

Assembly members:

Assembly members:
WSK3, polymer, 103 residues, 11404.746 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-24a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
WSK3: SADHEREAQKAEEELQKVLE EASKKAVEAERGAPGAALIS YPDAIWWSVETATTVGYGDR YPVTEEGRKVAEQVMKAGIE VFALVTAALATDFVRREEER RGH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	411
15N chemical shifts	113
1H chemical shifts	744

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_2	1
3	entity_3	1
4	entity_4	1

Entities:

Entity 1, entity_1 103 residues - 11404.746 Da.

This is the water-soluble analogue of transmembrane domain of potassium channel KcsA

1

SER

ALA

ASP

HIS

GLU

ARG

GLU

ALA

GLN

LYS

2

ALA

GLU

LEU

GLN

LYS

VAL

LEU

GLU

3

GLU

ALA

SER

LYS

ALA

VAL

GLU

ALA

GLU

4

ARG

GLY

ALA

PRO

GLY

ALA

LEU

ILE

SER

5

TYR

PRO

ASP

ALA

ILE

TRP

SER

VAL

GLU

6

THR

ALA

THR

VAL

GLY

TYR

GLY

ASP

ARG

7

TYR

PRO

VAL

THR

GLU

GLY

ARG

LYS

VAL

8

ALA

GLU

GLN

VAL

MET

LYS

ALA

GLY

ILE

GLU

9

VAL

PHE

ALA

LEU

VAL

THR

ALA

LEU

ALA

10

THR

ASP

PHE

VAL

ARG

GLU

ARG

11

ARG

GLY

HIS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	wsk3_13C15N	isotropic	sample_conditions_1
3D CBCA(CO)NH	wsk3_13C15N	isotropic	sample_conditions_1
3D HNCO	wsk3_13C15N	isotropic	sample_conditions_1
3D HNCA	wsk3_13C15N	isotropic	sample_conditions_1
3D HNCACB	wsk3_13C15N	isotropic	sample_conditions_1
3D HCCH-TOCSY	wsk3_13C15N	isotropic	sample_conditions_1
T1, T2, hetNOE	wsk3_13C15N	isotropic	sample_conditions_1
R2 dispersion	wsk3_13C15N	isotropic	sample_conditions_1
diffusion measurement	wsk3_13C15N	isotropic	sample_conditions_1

BMRB Entry 15677

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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