BMRB Entry 15642

Click here to enlarge.

PDB ID: 2k06
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15642
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solution structure of the aminoterminal domain of E. coli NusG
Deposition date:
2008-01-25
Original release date:
2009-06-10
Authors:
Schweimer, Kristian; Scheckenhofer, Ulrich; Roesch, Paul
Citation:

Citation: Mooney, Rachel Anne; Schweimer, Kristian; Roesch, Paul; Gottesman, Max; Landick, Robert. "Two structurally independent domains of E. coli NusG create regulatory plasticity via distinct interactions with RNA polymerase and regulators."  J. Mol. Biol. 391, 341-358 (2009).
PubMed: 19500594

Assembly members:

Assembly members:
NusG, polymer, 123 residues, 14096.366 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NusG: MSEAPKKRWYVVQAFSGFEG RVATSLREHIKLHNMEDLFG EVMVPTEEVVEIRGGQRRKS ERKFFPGYVLVQMVMNDASW HLVRSVPRVMGFIGGTSDRP APISDKEVDAIMNRLQQVGD KPR

Data sets:
Data typeCount
13C chemical shifts498
15N chemical shifts122
1H chemical shifts790

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NusG1

Entities:

Entity 1, NusG 123 residues - 14096.366 Da.

1   METSERGLUALAPROLYSLYSARGTRPTYR
2   VALVALGLNALAPHESERGLYPHEGLUGLY
3   ARGVALALATHRSERLEUARGGLUHISILE
4   LYSLEUHISASNMETGLUASPLEUPHEGLY
5   GLUVALMETVALPROTHRGLUGLUVALVAL
6   GLUILEARGGLYGLYGLNARGARGLYSSER
7   GLUARGLYSPHEPHEPROGLYTYRVALLEU
8   VALGLNMETVALMETASNASPALASERTRP
9   HISLEUVALARGSERVALPROARGVALMET
10   GLYPHEILEGLYGLYTHRSERASPARGPRO
11   ALAPROILESERASPLYSGLUVALASPALA
12   ILEMETASNARGLEUGLNGLNVALGLYASP
13   LYSPROARG

Related Database Links:

BMRB 15490
PDB 2JVV 2K06
DBJ BAB38328 BAE73404 BAE77338 BAG79793 BAH61119
EMBL CAD09492 CAG73137 CAH19518 CAL10413 CAL22339
GB AAA24622 AAC43080 AAC76956 AAF33495 AAG59178
PIR AB0934
REF NP_312932 NP_418409 NP_457922 NP_463017 NP_709777
SP P0AA01 P0AA02 P0AA03 P0AFG0 P0AFG1
AlphaFold P0AA01 P0AA02 P0AA03 P0AFG0 P0AFG1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks