BMRB Entry 15487

Click here to enlarge.

PDB ID: 2jvr
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15487
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Segmental Isotope Labeling of Npl3p
Deposition date:
2007-09-25
Original release date:
2008-06-26
Authors:
Skrisovska, Lenka; Allain, Frederic
Citation:

Citation: Skrisovska, Lenka; Allain, Frederic. "Improved segmental isotope labeling methods for the NMR study of multidomain or large proteins: application to the RRMs of Npl3p and hnRNP L"  J. Mol. Biol. 375, 151-164 (2008).
PubMed: 17936301

Assembly members:

Assembly members:
Npl3p_RRM2, polymer, 111 residues, 12545 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Npl3p_RRM2: MGSSHHHHHHSSGLVPRGSH MSKLPAKRYRITMKNLPEGC SWQDLKDLARENSLETTFSS VNTRDFDGTGALEFPSEEIL VEALERLNNIEFRGSVITVE RDDNPPPIRRS

Data sets:
Data typeCount
13C chemical shifts230
15N chemical shifts82
1H chemical shifts501

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Npl3p RRM21

Entities:

Entity 1, Npl3p RRM2 111 residues - 12545 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METSERLYSLEUPROALALYSARGTYRARG
4   ILETHRMETLYSASNLEUPROGLUGLYCYS
5   SERTRPGLNASPLEULYSASPLEUALAARG
6   GLUASNSERLEUGLUTHRTHRPHESERSER
7   VALASNTHRARGASPPHEASPGLYTHRGLY
8   ALALEUGLUPHEPROSERGLUGLUILELEU
9   VALGLUALALEUGLUARGLEUASNASNILE
10   GLUPHEARGGLYSERVALILETHRVALGLU
11   ARGASPASPASNPROPROPROILEARGARG
12   SER

Related Database Links:

PDB 2JVR 2OSR
DBJ GAA22648
EMBL CAA46817 CAA50291 CAY78932
GB AAA34818 AAB64865 AHY75384 AJP38110 AJU58232
REF NP_010720
SP Q01560
TPG DAA12270
AlphaFold Q01560

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks