BMRB Entry 15478

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PDB ID: 2jvf
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15478
MolProbity Validation Chart

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Title:
1H and 15N resonance assignment of M7, a computationally-designed artificial protein
Deposition date:
2007-09-19
Original release date:
2008-06-25
Authors:
Stordeur, Claudius; Dalluege, Roman; Birkenmeier, Olaf; Wienk, Hans; Rudolph, Rainer; Lange, Christian; Luecke, Christian
Citation:

Citation: Stordeur, Claudius; Dalluege, Roman; Birkenmeier, Olaf; Wienk, Hans; Rudolph, Rainer; Lange, Christian; Luecke, Christian. "The NMR solution structure of the artificial protein M7 matches the computationally designed model"  Proteins 72, 1104-1107 (2008).
PubMed: 18498106

Assembly members:

Assembly members:
M7, polymer, 96 residues, 10820.4 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not applicable

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
M7: GSHMKVDITIKIQRDGQEIE IDIRVSTGKELERALQELEK ALARAGARNVQITISAENDE QAKELLELIARLLQKLGYKD INVRVNGTEVKIEVRV

Data sets:
Data typeCount
15N chemical shifts111
1H chemical shifts728

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1M71

Entities:

Entity 1, M7 96 residues - 10820.4 Da.

The first four residues GSHM represent a cloning artifact; the protein sequence starts at Lys1.

1   GLYSERHISMETLYSVALASPILETHRILE
2   LYSILEGLNARGASPGLYGLNGLUILEGLU
3   ILEASPILEARGVALSERTHRGLYLYSGLU
4   LEUGLUARGALALEUGLNGLULEUGLULYS
5   ALALEUALAARGALAGLYALAARGASNVAL
6   GLNILETHRILESERALAGLUASNASPGLU
7   GLNALALYSGLULEULEUGLULEUILEALA
8   ARGLEULEUGLNLYSLEUGLYTYRLYSASP
9   ILEASNVALARGVALASNGLYTHRGLUVAL
10   LYSILEGLUVALARGVAL

Related Database Links:

PDB 2JVF

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