BMRB Entry 15473

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for 8M Urea Denatured State of dSUMO-3(dSMT3)
Published: 2007-09-19

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15473

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for 8M Urea Denatured State of dSUMO-3(dSMT3)

Deposition date:

2007-09-17

Original release date:

2007-09-19

Authors:

Kumar, Dinesh; Misra, Jyoti; Chugh, Jeetender; Sharma, Shilpy

Citation:

Citation: Kumar, Dinesh; Kumar, Ashutosh; Misra, Jyoti Ranjan; Chugh, Jeetender; Sharma, Shilpy; Hosur, Ramakrishna. "1H, 15N, 13C resonance assignment of folded and 8 M urea-denatured state of SUMO from Drosophila melanogaster" Biomol. NMR Assignments 2, 13-15 (2008).
PubMed: 19636913

Assembly members:

Assembly members:
dSUMO, polymer, 90 residues, 9972.2 Da.

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX4T1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
dSUMO: GSMSDEKKGGETEHINLKVL GQDNAVVQFKIKKHTPLRKL MNAYCDRAGLSMQVVRFRFD GQPINENDTPTSLEMEEGDT IEVYQQQTGG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	261
15N chemical shifts	90
1H chemical shifts	410

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	dSUMO	1

Entities:

Entity 1, dSUMO 90 residues - 9972.2 Da.

1	GLY	SER	MET	SER	ASP	GLU	LYS	LYS	GLY	GLY
2	GLU	THR	GLU	HIS	ILE	ASN	LEU	LYS	VAL	LEU
3	GLY	GLN	ASP	ASN	ALA	VAL	VAL	GLN	PHE	LYS
4	ILE	LYS	LYS	HIS	THR	PRO	LEU	ARG	LYS	LEU
5	MET	ASN	ALA	TYR	CYS	ASP	ARG	ALA	GLY	LEU
6	SER	MET	GLN	VAL	VAL	ARG	PHE	ARG	PHE	ASP
7	GLY	GLN	PRO	ILE	ASN	GLU	ASN	ASP	THR	PRO
8	THR	SER	LEU	GLU	MET	GLU	GLU	GLY	ASP	THR
9	ILE	GLU	VAL	TYR	GLN	GLN	GLN	THR	GLY	GLY

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNN	sample_1	isotropic	sample_conditions_1
3D HN(C)N	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1

PDB	2K1F
EMBL	CBA18485
GB	AAD19219 AAF31702 AAF52470 AAL28638 ALC38481
REF	NP_001303312 NP_477411 XP_001355784 XP_001650444 XP_001962391

BMRB Entry 15473

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	GLY	SER	MET	SER	ASP	GLU	LYS	LYS	GLY	GLY
2	GLU	THR	GLU	HIS	ILE	ASN	LEU	LYS	VAL	LEU
3	GLY	GLN	ASP	ASN	ALA	VAL	VAL	GLN	PHE	LYS
4	ILE	LYS	LYS	HIS	THR	PRO	LEU	ARG	LYS	LEU
5	MET	ASN	ALA	TYR	CYS	ASP	ARG	ALA	GLY	LEU
6	SER	MET	GLN	VAL	VAL	ARG	PHE	ARG	PHE	ASP
7	GLY	GLN	PRO	ILE	ASN	GLU	ASN	ASP	THR	PRO
8	THR	SER	LEU	GLU	MET	GLU	GLU	GLY	ASP	THR
9	ILE	GLU	VAL	TYR	GLN	GLN	GLN	THR	GLY	GLY

1	GLY	SER	MET	SER	ASP	GLU	LYS	LYS	GLY	GLY
2	GLU	THR	GLU	HIS	ILE	ASN	LEU	LYS	VAL	LEU
3	GLY	GLN	ASP	ASN	ALA	VAL	VAL	GLN	PHE	LYS
4	ILE	LYS	LYS	HIS	THR	PRO	LEU	ARG	LYS	LEU
5	MET	ASN	ALA	TYR	CYS	ASP	ARG	ALA	GLY	LEU
6	SER	MET	GLN	VAL	VAL	ARG	PHE	ARG	PHE	ASP
7	GLY	GLN	PRO	ILE	ASN	GLU	ASN	ASP	THR	PRO
8	THR	SER	LEU	GLU	MET	GLU	GLU	GLY	ASP	THR
9	ILE	GLU	VAL	TYR	GLN	GLN	GLN	THR	GLY	GLY

1	GLY	SER	MET	SER	ASP	GLU	LYS	LYS	GLY	GLY
2	GLU	THR	GLU	HIS	ILE	ASN	LEU	LYS	VAL	LEU
3	GLY	GLN	ASP	ASN	ALA	VAL	VAL	GLN	PHE	LYS
4	ILE	LYS	LYS	HIS	THR	PRO	LEU	ARG	LYS	LEU
5	MET	ASN	ALA	TYR	CYS	ASP	ARG	ALA	GLY	LEU
6	SER	MET	GLN	VAL	VAL	ARG	PHE	ARG	PHE	ASP
7	GLY	GLN	PRO	ILE	ASN	GLU	ASN	ASP	THR	PRO
8	THR	SER	LEU	GLU	MET	GLU	GLU	GLY	ASP	THR
9	ILE	GLU	VAL	TYR	GLN	GLN	GLN	THR	GLY	GLY