BMRB Entry 15451

Name: GABPa OST domain
Published: 2008-07-28

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PDB ID: 2juo
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15451
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

GABPa OST domain

Deposition date:

2007-08-31

Original release date:

2008-07-28

Authors:

Kang, Hyun-Seo; Nelson, Mary; Mackereth, Cameron; Schaerpf, Manuela; Graves, Barbara; McIntosh, Lawrence

Citation:

Citation: Kang, Hyun-Seo; Nelson, Mary; Mackereth, Cameron; Schaerpf, Manuela; Graves, Barbara; McIntosh, Lawrence. "Identification and structural characterization of a CBP/p300-binding domain from the ETS family transcription factor GABP alpha" J. Mol. Biol. 377, 636-646 (2008).
PubMed: 18295234

Assembly members:

Assembly members:
OST_(On-SighT), polymer, 89 residues, 9979.494 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
OST_(On-SighT): MAECVSQAIDINEPIGNLKK LLEPRLQCSLDAHEICLQDI QLDPDRSLFDQGVKTDGTVQ LSVQVISYQGMEPKLNILEI VKTAETVEW

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
coupling_constants
- coupling_constant_list_1
heteronucl_NOEs
- heteronuclear_noe_list_1
heteronucl_T1_relaxation
- heteronuclear_T1_list_1
heteronucl_T2_relaxation
- heteronuclear_T2_list_1
order_parameters
- order_parameter_list_1
RDCs
- RDC_list_1

Data type	Count
13C chemical shifts	397
15N chemical shifts	98
1H chemical shifts	628
coupling constants	57
heteronuclear NOE values	77
order parameters	77
residual dipolar couplings	50
T1 relaxation values	78
T2 relaxation values	78

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	OST_(On-SighT)	1

Entities:

Entity 1, OST_(On-SighT) 89 residues - 9979.494 Da.

residues 34-121 from murine GABPa plus C-terminal Trp

1

MET

ALA

GLU

CYS

VAL

SER

GLN

ALA

ILE

ASP

2

ILE

ASN

GLU

PRO

ILE

GLY

ASN

LEU

LYS

3

LEU

GLU

PRO

ARG

LEU

GLN

CYS

SER

LEU

4

ASP

ALA

HIS

GLU

ILE

CYS

LEU

GLN

ASP

ILE

5

GLN

LEU

ASP

PRO

ASP

ARG

SER

LEU

PHE

ASP

6

GLN

GLY

VAL

LYS

THR

ASP

GLY

THR

VAL

GLN

7

LEU

SER

VAL

GLN

VAL

ILE

SER

TYR

GLN

GLY

8

MET

GLU

PRO

LYS

LEU

ASN

ILE

LEU

GLU

ILE

9

VAL

LYS

THR

ALA

GLU

THR

VAL

GLU

TRP

Samples:

13C_15N_GABPa_OST: sodium phosphate 20 mM; sodium chloride 20 mM; GABPa OST, [U-100% 13C; U-100% 15N], 1 mM; DTT 2 mM

15N_GABPa_OST: sodium phosphate 20 mM; sodium chloride 50 mM; GABPa OST, [U-100% 15N], 1 mM; DTT 2 mM

15N_10%_13C_GABPa_OST: sodium phosphate 20 mM; sodium chloride 50 mM; GABPa OST, [U-10% 13C; U-100% 15N], 1 mM; DTT 2 mM

sample_conditions: pH: 7.0; temperature: 298.0 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	15N_GABPa_OST	isotropic	sample_conditions
2D 1H-13C HSQC	15N_10%_13C_GABPa_OST	isotropic	sample_conditions
3D CBCA(CO)NH	13C_15N_GABPa_OST	isotropic	sample_conditions
3D HNCO	13C_15N_GABPa_OST	isotropic	sample_conditions
3D HNCA	13C_15N_GABPa_OST	isotropic	sample_conditions
3D HNCACB	13C_15N_GABPa_OST	isotropic	sample_conditions
3D 1H-15N NOESY	15N_GABPa_OST	isotropic	sample_conditions
3D 1H-15N TOCSY	13C_15N_GABPa_OST	isotropic	sample_conditions
3D HCCH-TOCSY	13C_15N_GABPa_OST	isotropic	sample_conditions
simultaneous CN NOESY	13C_15N_GABPa_OST	isotropic	sample_conditions
EZ-HMQC-NH2	13C_15N_GABPa_OST	isotropic	sample_conditions
N-CG spin echo	13C_15N_GABPa_OST	isotropic	sample_conditions
CO-CG spin echo	13C_15N_GABPa_OST	isotropic	sample_conditions
15N IPAP	15N_GABPa_OST	anisotropic	sample_conditions
3D HNHB	15N_GABPa_OST	isotropic	sample_conditions
T1 T2 HNNOE	15N_GABPa_OST	isotropic	sample_conditions
3D HNHA	15N_GABPa_OST	isotropic	sample_conditions

Software:

ARIA v1.2, Brunger, Adams, Clore, Gros, Nilges and Read, Linge, O'Donoghue and Nilges - chemical shift assignment, refinement, structure solution

SPARKY v3.11, Goddard - chemical shift assignment, collection, peak picking

TALOS, Cornilescu, Delaglio and Bax - data analysis

Molmol, Koradi, Billeter and Wuthrich - data analysis, geometry optimization, structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

Varian Unity 500 MHz
Varian INOVA 600 MHz
Varian INOVA 800 MHz

PDB	2JUO
DBJ	BAE24181 BAE26442 BAE26687 BAE28337 BAE29575
GB	AAA53030 AAH52448 AAO27832 EDK98317 EDM10625
REF	NP_001102311 NP_032091 XP_001363875 XP_002716818 XP_003467234
SP	Q00422
AlphaFold	Q00422