BMRB Entry 15385

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PDB ID: 2jt0
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15385
MolProbity Validation Chart

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Title:
Solution structure of F104W cardiac troponin C
Deposition date:
2007-07-17
Original release date:
2007-08-15
Authors:
Wang, Xu; Mercier, Pascal; Letourneau, Paul-Jean; Sykes, Brian
Citation:

Citation: Wang, Xu; Mercier, Pascal; Letourneau, Paul-Jean; Sykes, Brian. "Effects of Phe-to-Trp mutation and fluorotryptophan incorporation on the solution structure of cardiac troponin C, and analysis of its suitability as a potential probe for in situ NMR studies"  Protein Sci. 14, 2447-2460 (2005).
PubMed: 16131667

Assembly members:

Assembly members:
F104W, polymer, 161 residues, 18428.521 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
F104W: MDDIYKAAVEQLTEEQKNEF KAAFDIFVLGAEDGSISTKE LGKVMRMLGQNPTPEELQEM IDEVDEDGSGTVDFDEFLVM MVRSMKDDSKGKSEEELSDL FRMWDKNADGYIDLDELKIM LQATGETITEDDIEELMKDG DKNNDGRIDYDEFLEFMKGV E

Data sets:
Data typeCount
13C chemical shifts513
15N chemical shifts158
1H chemical shifts1057

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1F104W cTnC1
2Calcium ion2

Entities:

Entity 1, F104W cTnC 161 residues - 18428.521 Da.

This protein is a version of cardiac troponin C with F104 mutated to W

1   METASPASPILETYRLYSALAALAVALGLU
2   GLNLEUTHRGLUGLUGLNLYSASNGLUPHE
3   LYSALAALAPHEASPILEPHEVALLEUGLY
4   ALAGLUASPGLYSERILESERTHRLYSGLU
5   LEUGLYLYSVALMETARGMETLEUGLYGLN
6   ASNPROTHRPROGLUGLULEUGLNGLUMET
7   ILEASPGLUVALASPGLUASPGLYSERGLY
8   THRVALASPPHEASPGLUPHELEUVALMET
9   METVALARGSERMETLYSASPASPSERLYS
10   GLYLYSSERGLUGLUGLULEUSERASPLEU
11   PHEARGMETTRPASPLYSASNALAASPGLY
12   TYRILEASPLEUASPGLULEULYSILEMET
13   LEUGLNALATHRGLYGLUTHRILETHRGLU
14   ASPASPILEGLUGLULEUMETLYSASPGLY
15   ASPLYSASNASNASPGLYARGILEASPTYR
16   ASPGLUPHELEUGLUPHEMETLYSGLYVAL
17   GLU

Entity 2, Calcium ion - Ca - 40.078 Da.

1   CA

Related Database Links:

BMRB 15388 15400 15427 16190 17103 19789 25034 25035 25120 25495 25797
PDB 1AJ4 1AP4 1DTL 1J1D 1J1E 1LXF 1MXL 1SPY 1WRK 1WRL 2CTN 2JT0 2JT3 2JT8 2JTZ 2KFX 2KRD 2L1R 2MKP 2MZP 3SD6 3SWB 4GJE
DBJ BAG36483
EMBL CAA30736 CAG46663 CAG46683
GB AAA36772 AAA37492 AAA37493 AAB91994 AAH30244
PIR TPHUCC
PRF 1510257A 750650A
REF NP_001029277 NP_001029523 NP_001123715 NP_001272501 NP_003271
SP P02591 P19123 P63315 P63316 P63317
TPG DAA16908
AlphaFold P02591 P19123 P63315 P63316 P63317

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