BMRB Entry 15383

Assembly:

Entities:

Entity 1, ClpC N-domain 146 residues - Formula weight is not available

Residue H1 is a cloning artifact from Factor Xa cleavage. NMR assignments start from the first M residue. Hereafter, the residue number should be H-1 and M1.

Samples:

sample_1: NClpCR, [U-13C; U-15N], 0.75 ± 0.25 mM; MES 20 mM; sodium chloride 50 mM; EDTA 2 mM; D2O 10%; H2O 90%

sample_2: NClpCR, [U-15N], 0.75 ± 0.25 mM; MES 20 mM; sodium chloride 50 mM; EDTA 2 mM; D2O 10%; H2O 90%

sample_3: NClpCR, [U-10% 13C], 0.75 ± 0.25 mM; MES 20 mM; sodium chloride 50 mM; EDTA 2 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 50 mM; pH: 5.5; pressure: 1 atm; temperature: 305 K

Experiments:

Software:

NMRView, Johnson, One Moon Scientific - data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

• Varian INOVA 800 MHz
• Varian INOVA 600 MHz
• Varian INOVA 500 MHz