BMRB Entry 15373

Click here to enlarge.

PDB ID: 2jsp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15373
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
The prokaryotic Cys2His2 zinc finger adopts a novel fold as revealed by the NMR structure of A. tumefaciens Ros DNA binding domain
Deposition date:
2007-07-10
Original release date:
2008-03-13
Authors:
Malgieri, Gaetano
Citation:

Citation: Malgieri, Gaetano; Russo, L.; Esposito, S.; Baglivo, I.; Zaccaro, L.; Pedone, E.; Di Blasio, B.; Isernia, C.; Pedone, P.; Fattorusso, R.. "The prokaryotic Cys2His2 zinc finger adopts a novel fold as revealed by the NMR structure of A.tumefaciens Ros DNA binding domain"  Proc. Natl. Acad. Sci. U.S.A. 104, 17341-17346 (2007).
PubMed: 17956987

Assembly members:

Assembly members:
Ros87_polypeptide, polymer, 87 residues, 9959.560 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Agrobacterium tumefaciens   Taxonomy ID: 358   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Agrobacterium tumefaciens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-11d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ros87_polypeptide: AVNVEKQKPAVSVRKSVQDD HIVCLECGGSFKSLKRHLTT HHSMTPEEYREKWDLPVDYP MVAPAYAEARSRLAKEMGLG QRRKANR

Data sets:
Data typeCount
13C chemical shifts341
15N chemical shifts85
1H chemical shifts505

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ros87 polypeptide1
2ZINC ION2

Entities:

Entity 1, Ros87 polypeptide 87 residues - 9959.560 Da.

1   ALAVALASNVALGLULYSGLNLYSPROALA
2   VALSERVALARGLYSSERVALGLNASPASP
3   HISILEVALCYSLEUGLUCYSGLYGLYSER
4   PHELYSSERLEULYSARGHISLEUTHRTHR
5   HISHISSERMETTHRPROGLUGLUTYRARG
6   GLULYSTRPASPLEUPROVALASPTYRPRO
7   METVALALAPROALATYRALAGLUALAARG
8   SERARGLEUALALYSGLUMETGLYLEUGLY
9   GLNARGARGLYSALAASNARG

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Related Database Links:

PDB 2JSP
EMBL CAA58116 CDI07817 CDN91482
GB AAA22106 AAK86720 ADY63837 AHK00781 AKC06614
REF NP_353935 WP_003497181 WP_003509257 WP_003512037 WP_004440665
SP P55324 Q04152
AlphaFold P55324 Q04152

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks