BMRB Entry 15312

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PDB ID: 2jr0
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15312
MolProbity Validation Chart

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Title:
Solution structure of NusB from Aquifex Aeolicus
Deposition date:
2007-06-18
Original release date:
2008-02-28
Authors:
Das, Ranabir; Loss, Sandra; Li, Jess; Tarasov, Sergey; Wingfield, Paul; Waugh, David; Byrd, Robert; Altieri, Amanda
Citation:

Citation: Das, Ranabir; Loss, Sandra; Li, Jess; Waugh, David; Tarasov, Sergey; Wingfield, Paul; Byrd, Robert; Altieri, Amanda. "Structural biophysics of the NusB:NusE antitermination complex"  J. Mol. Biol. 376, 705-720 (2008).
PubMed: 18177898

Assembly members:

Assembly members:
NusB, polymer, 148 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 63363   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Aquifex aeolicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: peT3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NusB: MRYRKGARDTAFLVLYRWDL RGENPGELFKEVVEEKNIKN KDAYEYAKKLVDTAVRHIEE IDSIIEKHLKGWSIDRLGYV ERNALRLGVAELIFLKSKEP GRVFIDIVDLVKKYADEKAG KFVNGVLSAIYKAYITSSKE EKPSLKSE

Data sets:
Data typeCount
13C chemical shifts656
15N chemical shifts143
1H chemical shifts1030

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Aquifex NusB monomer1

Entities:

Entity 1, Aquifex NusB monomer 148 residues - Formula weight is not available

1   METARGTYRARGLYSGLYALAARGASPTHR
2   ALAPHELEUVALLEUTYRARGTRPASPLEU
3   ARGGLYGLUASNPROGLYGLULEUPHELYS
4   GLUVALVALGLUGLULYSASNILELYSASN
5   LYSASPALATYRGLUTYRALALYSLYSLEU
6   VALASPTHRALAVALARGHISILEGLUGLU
7   ILEASPSERILEILEGLULYSHISLEULYS
8   GLYTRPSERILEASPARGLEUGLYTYRVAL
9   GLUARGASNALALEUARGLEUGLYVALALA
10   GLULEUILEPHELEULYSSERLYSGLUPRO
11   GLYARGVALPHEILEASPILEVALASPLEU
12   VALLYSLYSTYRALAASPGLULYSALAGLY
13   LYSPHEVALASNGLYVALLEUSERALAILE
14   TYRLYSALATYRILETHRSERSERLYSGLU
15   GLULYSPROSERLEULYSSERGLU

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