BMRB Entry 15257

Name: Structural basis of RsmA/CsrA RNA recognition: Structure of RsmE bound to the Shine-Dalgarno sequence of hcnA mRNA
Published: 2007-09-12

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PDB ID: 2jpp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15257
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structural basis of RsmA/CsrA RNA recognition: Structure of RsmE bound to the Shine-Dalgarno sequence of hcnA mRNA

Deposition date:

2007-05-21

Original release date:

2007-09-12

Authors:

Schubert, Mario; Lapouge, Karine; Duss, Olivier; Oberstrass, Florian; Jelesarov, Ilian; Haas, Dieter; Allain, Frederic

Citation:

Citation: Schubert, Mario; Lapouge, Karine; Duss, Olivier; Oberstrass, Florian; Jelesarov, Ilian; Haas, Dieter; Allain, Frederic. "Molecular basis of messenger RNA recognition by the specific bacterial repressing clamp RsmA/CsrA." Nat. Struct. Mol. Biol. 14, 807-813 (2007).
PubMed: 17704818

Assembly members:

Assembly members:
hncA_mRNA_SD_20mer, polymer, 20 residues, 6437.938 Da.
RsmE, polymer, 70 residues, 5851.832 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: 294 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas fluorescens

Experimental source:

Experimental source: Production method: in vitro transcription Host organism: Pseudomonas fluorescens Vector: not applicable

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hncA_mRNA_SD_20mer: GGGCUUCACGGAUGAAGCCC
RsmE: MLILTRKVGESINIGDDITI TILGVSGQQVRIGINAPKDV AVHREEIYQRIQAGLTAPDK RETPHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	340
15N chemical shifts	77
1H chemical shifts	625

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	20 mer, 1	1
2	20 mer, 2	1
3	RsmE A	2
4	RsmE B	2

Entities:

Entity 1, 20 mer, 1 20 residues - 6437.938 Da.

1		G	G	G	C	U	U	C	A	C	G
2		G	A	U	G	A	A	G	C	C	C

Entity 2, RsmE A 70 residues - 5851.832 Da.

1	MET	LEU	ILE	LEU	THR	ARG	LYS	VAL	GLY	GLU
2	SER	ILE	ASN	ILE	GLY	ASP	ASP	ILE	THR	ILE
3	THR	ILE	LEU	GLY	VAL	SER	GLY	GLN	GLN	VAL
4	ARG	ILE	GLY	ILE	ASN	ALA	PRO	LYS	ASP	VAL
5	ALA	VAL	HIS	ARG	GLU	GLU	ILE	TYR	GLN	ARG
6	ILE	GLN	ALA	GLY	LEU	THR	ALA	PRO	ASP	LYS
7	ARG	GLU	THR	PRO	HIS	HIS	HIS	HIS	HIS	HIS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_3	isotropic	sample_conditions_1
3D HNCA	sample_3	isotropic	sample_conditions_1
3D HNCACB	sample_3	isotropic	sample_conditions_1
3D HNCO	sample_3	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_3	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_3	isotropic	sample_conditions_1
3D HN(CO)CA	sample_3	isotropic	sample_conditions_1

BMRB	19534 19544 19546 19547 19548 19549
PDB	2JPP 2MF0 2MF1 2MFC 2MFE 2MFF 2MFG 2MFH
DBJ	BAO61455 BAQ73744 BAQ80031
GB	AAT27429 AAY91370 AEL31265 AGL83913 AIC19187
REF	WP_007920550 WP_017337657 WP_045057924 WP_057444113

BMRB Entry 15257

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	LEU	ILE	LEU	THR	ARG	LYS	VAL	GLY	GLU
2	SER	ILE	ASN	ILE	GLY	ASP	ASP	ILE	THR	ILE
3	THR	ILE	LEU	GLY	VAL	SER	GLY	GLN	GLN	VAL
4	ARG	ILE	GLY	ILE	ASN	ALA	PRO	LYS	ASP	VAL
5	ALA	VAL	HIS	ARG	GLU	GLU	ILE	TYR	GLN	ARG
6	ILE	GLN	ALA	GLY	LEU	THR	ALA	PRO	ASP	LYS
7	ARG	GLU	THR	PRO	HIS	HIS	HIS	HIS	HIS	HIS

1	MET	LEU	ILE	LEU	THR	ARG	LYS	VAL	GLY	GLU
2	SER	ILE	ASN	ILE	GLY	ASP	ASP	ILE	THR	ILE
3	THR	ILE	LEU	GLY	VAL	SER	GLY	GLN	GLN	VAL
4	ARG	ILE	GLY	ILE	ASN	ALA	PRO	LYS	ASP	VAL
5	ALA	VAL	HIS	ARG	GLU	GLU	ILE	TYR	GLN	ARG
6	ILE	GLN	ALA	GLY	LEU	THR	ALA	PRO	ASP	LYS
7	ARG	GLU	THR	PRO	HIS	HIS	HIS	HIS	HIS	HIS

1	MET	LEU	ILE	LEU	THR	ARG	LYS	VAL	GLY	GLU
2	SER	ILE	ASN	ILE	GLY	ASP	ASP	ILE	THR	ILE
3	THR	ILE	LEU	GLY	VAL	SER	GLY	GLN	GLN	VAL
4	ARG	ILE	GLY	ILE	ASN	ALA	PRO	LYS	ASP	VAL
5	ALA	VAL	HIS	ARG	GLU	GLU	ILE	TYR	GLN	ARG
6	ILE	GLN	ALA	GLY	LEU	THR	ALA	PRO	ASP	LYS
7	ARG	GLU	THR	PRO	HIS	HIS	HIS	HIS	HIS	HIS