BMRB Entry 15230
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR15230
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Prasannan, Sunil; Fenwick, R. Bryn; Campbell, Louise; Evetts, Katrina; Nietlispach, Daniel; Owen, Darerca; Mott, Helen. "1H, 13C and 15N resonance assignments for the small G protein RalB in its active conformation" Biomol. NMR Assignments 1, 147-149 (2007).
PubMed: 19636851
Assembly members:
RalB, polymer, 186 residues, Formula weight is not available
GNP, non-polymer, Formula weight is not available
MG, non-polymer, Formula weight is not available
WATER, water, 18.015 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET16b
- assigned_chemical_shifts
- heteronucl_NOEs
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
- order_parameters
| Data type | Count |
| 13C chemical shifts | 776 |
| 15N chemical shifts | 178 |
| 1H chemical shifts | 1243 |
| T1 relaxation values | 127 |
| T2 relaxation values | 127 |
| heteronuclear NOE values | 127 |
| order parameters | 127 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RalB | 1 |
| 2 | GMPPNP | 2 |
| 3 | MAGNESIUM ION | 3 |
| 4 | BOUND WATER, 1 | 4 |
| 5 | BOUND WATER, 2 | 4 |
Entities:
Entity 1, RalB 186 residues - Formula weight is not available
Residues 0 (H) from His-Tag. Residues Met1 to Ser11 not assigned, due to proteolysis
| 1 | HIS | MET | ALA | ALA | ASN | LYS | SER | LYS | GLY | GLN | ||||
| 2 | SER | SER | LEU | ALA | LEU | HIS | LYS | VAL | ILE | MET | ||||
| 3 | VAL | GLY | SER | GLY | GLY | VAL | GLY | LYS | SER | ALA | ||||
| 4 | LEU | THR | LEU | GLN | PHE | MET | TYR | ASP | GLU | PHE | ||||
| 5 | VAL | GLU | ASP | TYR | GLU | PRO | THR | LYS | ALA | ASP | ||||
| 6 | SER | TYR | ARG | LYS | LYS | VAL | VAL | LEU | ASP | GLY | ||||
| 7 | GLU | GLU | VAL | GLN | ILE | ASP | ILE | LEU | ASP | THR | ||||
| 8 | ALA | GLY | LEU | GLU | ASP | TYR | ALA | ALA | ILE | ARG | ||||
| 9 | ASP | ASN | TYR | PHE | ARG | SER | GLY | GLU | GLY | PHE | ||||
| 10 | LEU | LEU | VAL | PHE | SER | ILE | THR | GLU | HIS | GLU | ||||
| 11 | SER | PHE | THR | ALA | THR | ALA | GLU | PHE | ARG | GLU | ||||
| 12 | GLN | ILE | LEU | ARG | VAL | LYS | ALA | GLU | GLU | ASP | ||||
| 13 | LYS | ILE | PRO | LEU | LEU | VAL | VAL | GLY | ASN | LYS | ||||
| 14 | SER | ASP | LEU | GLU | GLU | ARG | ARG | GLN | VAL | PRO | ||||
| 15 | VAL | GLU | GLU | ALA | ARG | SER | LYS | ALA | GLU | GLU | ||||
| 16 | TRP | GLY | VAL | GLN | TYR | VAL | GLU | THR | SER | ALA | ||||
| 17 | LYS | THR | ARG | ALA | ASN | VAL | ASP | LYS | VAL | PHE | ||||
| 18 | PHE | ASP | LEU | MET | ARG | GLU | ILE | ARG | THR | LYS | ||||
| 19 | LYS | MET | SER | GLU | ASN | LYS |
Entity 2, GMPPNP - Formula weight is not available
| 1 | GNP |
Entity 3, MAGNESIUM ION - Formula weight is not available
| 1 | MG |
Entity 4, BOUND WATER, 1 - 18.015 Da.
| 1 | HOH |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks