BMRB Entry 15214

Name: MxiHCdelta5 backbone assignment
Published: 2007-09-12

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15214

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

MxiHCdelta5 backbone assignment

Deposition date:

2007-04-17

Original release date:

2007-09-12

Authors:

Zhang, Lingling

Citation:

Citation: Zhang, Lingling; Wang, Yu; Olive, Andrew; Smith, Nathan; Picking, William; De Guzman, Roberto; Picking, Wendy. "Identification of the MxiH needle protein residues responsible for anchoring invasion plasmid antigen D to the type III secretion needle tip" J. Biol. Chem. 282, 32144-32151 (2007).
PubMed: 17827155

Assembly members:

Assembly members:
MxiH, polymer, 86 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Shigella flexneri Taxonomy ID: 623 Superkingdom: Bacteria Kingdom: not available Genus/species: Shigella flexneri

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MxiH: MSVTVPNDDWTLSSLSETFD DGTQTLQGELTLALDKLAKN PSNPQLLAEYQSKLSEYTLY RNAQSNTVKVIKDVDAAILE HHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	50
15N chemical shifts	63
1H chemical shifts	63

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MxiH	1

Entities:

Entity 1, MxiH 86 residues - Formula weight is not available

1

MET

SER

VAL

THR

VAL

PRO

ASN

ASP

TRP

2

THR

LEU

SER

LEU

SER

GLU

THR

PHE

ASP

3

ASP

GLY

THR

GLN

THR

LEU

GLN

GLY

GLU

LEU

4

THR

LEU

ALA

LEU

ASP

LYS

LEU

ALA

LYS

ASN

5

PRO

SER

ASN

PRO

GLN

LEU

ALA

GLU

TYR

6

GLN

SER

LYS

LEU

SER

GLU

TYR

THR

LEU

TYR

7

ARG

ASN

ALA

GLN

SER

ASN

THR

VAL

LYS

VAL

8

ILE

LYS

ASP

VAL

ASP

ALA

ILE

LEU

GLU

9

HIS

Samples:

sample_1: MxiH, [U-13C; U-99% 15N], 0.7 mM; NaCl 10 mM; Na Phosphate 10 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz

PDB	2CA5
GB	EGW99644
REF	WP_001381656 WP_052993805