BMRB Entry 15128

Assembly:

Entities:

Entity 1, RGS domain 154 residues - 17728.135 Da.

The two residues at the extreme N-terminus (SM) are from the vector following TEV cleavage of the N-terminus hexahistidine purification tag.

Samples:

sample_1: RGS14, [U-95% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM; D2O 10%; DTT, [U-99% 2H], 1 mM

sample_2: RGS14, [U-95% 13C; U-95% 15N], 1.7 mM; sodium phosphate 20 mM; sodium chloride 50 mM; DTT, [U-99% 2H], 1 mM; D2O 10%

sample_3: RGS14, [U-95% 13C; U-95% 15N], 1.4 mM; sodium phosphate 20 mM; sodium chloride 50 mM; DTT, [U-99% 2H], 1 mM; D2O 10%

sample_4: RGS14, [U-95% 13C; U-95% 15N], 1.1 mM; sodium phosphate 20 mM; sodium chloride 50 mM; DTT, [U-99% 2H], 1 mM; D2O, [U-100% 2H], 100%; sodium azide 0.02%

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 297 K

sample_conditions_2: pH: 6; pressure: 1 atm; temperature: 297 K

sample_conditions_3: pH: 6.2; pressure: 1 atm; temperature: 297 K

sample_conditions_4: pH: 6; pressure: 1 atm; temperature: 297 K

Experiments:

Software:

xwinnmr v2.6 and 3.1, Bruker Biospin - collection, processing

SPARKY v3.100, Goddard - chemical shift assignment, data analysis, peak picking

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

X-PLOR NIH v2.14, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

CNSSOLVE v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

• Bruker DRX 600 MHz
• Bruker DMX 750 MHz