BMRB Entry 15114

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15114

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Title:
Solution structure of L8A mutant of HIV-1 myristoylated matrix protein
Deposition date:
2007-01-27
Original release date:
2007-03-06
Authors:
Saad, J.; Loeliger, E.; Luncsford, P.; Liriano, M.; Tai, J.; Kim, A.; Miller, J.; Joshi, A.; Freed, E.; Summers, M.
Citation:

Citation: Saad, J.; Loeliger, E.; Luncsford, P.; Liriano, M.; Tai, J.; Kim, A.; Miller, J.; Joshi, A.; Freed, E.; Summers, M.. "Point Mutations in the HIV-1 Matrix Protein Turn Off the Myristyl Switch."  J. Mol. Biol. 366, 574-585 (2007).
PubMed: 17188710

Assembly members:

Assembly members:
Gag_polyprotein, polymer, 131 residues, Formula weight is not available
MYRISTIC ACID, non-polymer, 228.371 Da.

Natural source:

Natural source:   Common Name: virus   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11 and pET19

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Gag_polyprotein: GARASVASGGELDKWEKIRL RPGGKKQYKLKHIVWASREL ERFAVNPGLLETSEGCRQIL GQLQPSLQTGSEELRSLYNT IAVLYCVHQRIDVKDTKEAL DKIEEEQNKSKKKAQQAAAD TGNNSQVSQNY

Data sets:
Data typeCount
13C chemical shifts431
15N chemical shifts129
1H chemical shifts771

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HIV-1 L8A-myrMA1
2myr2

Entities:

Entity 1, HIV-1 L8A-myrMA 131 residues - Formula weight is not available

1   GLYALAARGALASERVALALASERGLYGLY
2   GLULEUASPLYSTRPGLULYSILEARGLEU
3   ARGPROGLYGLYLYSLYSGLNTYRLYSLEU
4   LYSHISILEVALTRPALASERARGGLULEU
5   GLUARGPHEALAVALASNPROGLYLEULEU
6   GLUTHRSERGLUGLYCYSARGGLNILELEU
7   GLYGLNLEUGLNPROSERLEUGLNTHRGLY
8   SERGLUGLULEUARGSERLEUTYRASNTHR
9   ILEALAVALLEUTYRCYSVALHISGLNARG
10   ILEASPVALLYSASPTHRLYSGLUALALEU
11   ASPLYSILEGLUGLUGLUGLNASNLYSSER
12   LYSLYSLYSALAGLNGLNALAALAALAASP
13   THRGLYASNASNSERGLNVALSERGLNASN
14   TYR

Entity 2, myr - C14 H28 O2 - 228.371 Da.

1   MYR

Related Database Links:

BMRB 15116 18715 18716
PDB 1HIW 1L6N 1UPH 2GOL 2H3F 2H3I 2H3Q 2H3V 2H3Z 2HMX 2JMG 2LYA 2LYB 2NV3 4JMU
DBJ BAF34641 BAG48474
EMBL CBI61180 CBI61181 CBI61182 CBI61183 CBI61184
GB AAA44987 AAB00898 AAB60571 AAC28445 AAC29216
SP P12493 P12497
AlphaFold P12493 P12497

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks