BMRB Entry 15083

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PDB ID: 2o8k
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR15083
MolProbity Validation Chart

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Title:
NMR Structure of the Sigma-54 RpoN Domain Bound to the-24 Promoter Element
Deposition date:
2006-12-19
Original release date:
2007-10-17
Authors:
Doucleff, M.; Pelton, J.; Lee, P.; Wemmer, D.
Citation:

Citation: Doucleff, M.; Pelton, J.; Lee, P.; Nixon, B.; Wemmer, D.. "Structural basis of DNA recognition by the alternative sigma-factor, sigma54"  J. Mol. Biol. 369, 1070-1078 (2007).
PubMed: 17481658

Assembly members:

Assembly members:
NIRB_SIGMA-54_BINDING_SITE_NON-TEMPLATE_STRAND, polymer, 14 residues, 4364.794 Da.
NIRB_SIGMA-54_BINDING_SITE_TEMPLATE_STRAND, polymer, 14 residues, 4514.872 Da.
RNA_POLYMERASE_SIGMA_FACTOR_RPON, polymer, 63 residues, 7490.880 Da.

Natural source:

Natural source:   Common Name: Aquifex aeolicus   Taxonomy ID: 63363   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Aquifex aeolicus

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NIRB_SIGMA-54_BINDING_SITE_NON-TEMPLATE_STRAND: TTTTGGCACGTTTC
NIRB_SIGMA-54_BINDING_SITE_TEMPLATE_STRAND: GAAACGTGCCAAAA
RNA_POLYMERASE_SIGMA_FACTOR_RPON: HMLTQGELMKLIKEIVENED KRKPYSDQEIANILKEKGFK VARRTVAKYREMLGIPSSRE RRI

Data sets:
Data typeCount
13C chemical shifts208
15N chemical shifts61
1H chemical shifts663

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1-24 ELEMENT NON-TEMPLATE STRAND1
2-24 ELEMENT TEMPLATE STRAND2
3C-TERMINAL RPON DOMAIN3

Entities:

Entity 1, -24 ELEMENT NON-TEMPLATE STRAND 14 residues - 4364.794 Da.

1   DTDTDTDTDGDGDCDADCDG
2   DTDTDTDC

Entity 2, -24 ELEMENT TEMPLATE STRAND 14 residues - 4514.872 Da.

1   DGDADADADCDGDTDGDCDC
2   DADADADA

Entity 3, C-TERMINAL RPON DOMAIN 63 residues - 7490.880 Da.

1   HISMETLEUTHRGLNGLYGLULEUMETLYS
2   LEUILELYSGLUILEVALGLUASNGLUASP
3   LYSARGLYSPROTYRSERASPGLNGLUILE
4   ALAASNILELEULYSGLULYSGLYPHELYS
5   VALALAARGARGTHRVALALALYSTYRARG
6   GLUMETLEUGLYILEPROSERSERARGGLU
7   ARGARGILE

Related Database Links:

PDB 2AHQ 2O8K 2O9L
GB AAC06814
REF NP_213418 WP_010880356

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