BMRB Entry 15007

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15007
MolProbity Validation Chart

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Title:
Structural Studies on Plasmodium vivax Merozoite Surface Protein-1
Deposition date:
2006-10-30
Original release date:
2008-02-04
Authors:
Babon, Jeffrey; Morgan, William; Geoff, Kelly; John, Eccleston; James, Feeney; Anthony, Holder
Citation:

Citation: Babon, Jeffrey; Morgan, William; Kelly, Geoff; Eccleston, John; Feeney, James; Holder, Anthony. "Structural studies on Plasmodium vivax merozoite surface protein-1."  Mol. Biochem. Parasitol. 153, 31-40 (2007).
PubMed: 17343930

Assembly members:

Assembly members:
msp-1, polymer, 90 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: PLASMODIUM VIVAX   Taxonomy ID: 5855   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Plasmodium vivax

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-30Xa/LIC

Entity Sequences (FASTA):

Entity Sequences (FASTA):
msp-1: TMSSEHTCIDTNVPDNAACY RYLDGTEEWRCLLTFKEEGG KCVPASNVTCKDNNGGCAPE AECKMTDSNKIVCKCTKEGS EPLFEGVFCS

Data sets:
Data typeCount
13C chemical shifts301
15N chemical shifts87
1H chemical shifts487

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1msp-11

Entities:

Entity 1, msp-1 90 residues - Formula weight is not available

1   THRMETSERSERGLUHISTHRCYSILEASP
2   THRASNVALPROASPASNALAALACYSTYR
3   ARGTYRLEUASPGLYTHRGLUGLUTRPARG
4   CYSLEULEUTHRPHELYSGLUGLUGLYGLY
5   LYSCYSVALPROALASERASNVALTHRCYS
6   LYSASPASNASNGLYGLYCYSALAPROGLU
7   ALAGLUCYSLYSMETTHRASPSERASNLYS
8   ILEVALCYSLYSCYSTHRLYSGLUGLYSER
9   GLUPROLEUPHEGLUGLYVALPHECYSSER

Samples:

sample_1: msp-1, [U-100% 13C; U-100% 15N], 0.5 – 1.0 mM; k-phosphate 20 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.02 M; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, CD Schwieters, JJ Kuszewski, N Tjandra and GM Clore - structure solution

NMRPipe, F Delaglio, S Grzesiek, GW Vuister, G Zhu, J Pfeifer and A Bax - data analysis

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAJ41307 BAJ41308 BAJ41309 BAJ41310 BAJ41311
EMBL CAC82066 CAC82067 CAC82068 CAC82069 CAC82070
GB AAA29735 AAA63427 AAC37236 AAC37237 AAF91156
REF XP_001614842

Download HSQC peak lists in one of the following formats:
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