BMRB Entry 11609

Name: Solution structure of the isolated histone H2A-H2B heterodimer
Published: 2016-05-23

Click here to enlarge.

PDB ID: 2rvq
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11609
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structure of the isolated histone H2A-H2B heterodimer

Deposition date:

2016-03-28

Original release date:

2016-05-23

Authors:

Moriwaki, Yoshihito; Yamane, Tsutomu; Ohtomo, Hideaki; Ikeguchi, Mitsunori; Kurita, Jun-ichi; Sato, Masahiko; Nagadoi, Aritaka; Shimojo, Hideaki; Nishimura, Yoshifumi

Citation:

Citation: Moriwaki, Yoshihito; Yamane, Tsutomu; Ohtomo, Hideaki; Ikeguchi, Mitsunori; Kurita, Jun-ichi; Sato, Masahiko; Nagadoi, Aritaka; Shimojo, Hideaki; Nishimura, Yoshifumi. "Solution structure of the isolated histone H2A-H2B heterodimer" Sci. Rep. 6, 24999-24999 (2016).
PubMed: 27181506

Assembly members:

Assembly members:
entity_1, polymer, 133 residues, 14376.907 Da.
entity_2, polymer, 129 residues, 14146.588 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-23b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPGMSGRGKQGGKARAKAKT RSSRAGLQFPVGRVHRLLRK GNYSERVGAGAPVYLAAVLE YLTAEILELAGNAARDNKKT RIIPRHLQLAIRNDEELNKL LGRVTIAQGGVLPNIQAVLL PKKTESHHKAKGK
entity_2: GPGMPEPAKSAPAPKKGSKK AVTKAQKKDGKKRKRSRKES YSIYVYKVLKQVHPDTGISS KAMGIMNSFVNDIFERIAGE ASRLAHYNKRSTITSREIQT AVRLLLPGELAKHAVSEGTK AVTKYTSAK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list_2

Data type	Count
13C chemical shifts	736
15N chemical shifts	242
1H chemical shifts	243

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_2	2

Entities:

Entity 1, entity_1 133 residues - 14376.907 Da.

1

GLY

PRO

GLY

MET

SER

GLY

ARG

GLY

LYS

GLN

2

GLY

LYS

ALA

ARG

ALA

LYS

ALA

LYS

THR

3

ARG

SER

ARG

ALA

GLY

LEU

GLN

PHE

PRO

4

VAL

GLY

ARG

VAL

HIS

ARG

LEU

ARG

LYS

5

GLY

ASN

TYR

SER

GLU

ARG

VAL

GLY

ALA

GLY

6

ALA

PRO

VAL

TYR

LEU

ALA

VAL

LEU

GLU

7

TYR

LEU

THR

ALA

GLU

ILE

LEU

GLU

LEU

ALA

8

GLY

ASN

ALA

ARG

ASP

ASN

LYS

THR

9

ARG

ILE

PRO

ARG

HIS

LEU

GLN

LEU

ALA

10

ILE

ARG

ASN

ASP

GLU

LEU

ASN

LYS

LEU

11

LEU

GLY

ARG

VAL

THR

ILE

ALA

GLN

GLY

12

VAL

LEU

PRO

ASN

ILE

GLN

ALA

VAL

LEU

13

PRO

LYS

THR

GLU

SER

HIS

LYS

ALA

14

LYS

GLY

LYS

Entity 2, entity_2 129 residues - 14146.588 Da.

1

GLY

PRO

GLY

MET

PRO

GLU

PRO

ALA

LYS

SER

2

ALA

PRO

ALA

PRO

LYS

GLY

SER

LYS

3

ALA

VAL

THR

LYS

ALA

GLN

LYS

ASP

GLY

4

LYS

ARG

LYS

ARG

SER

ARG

LYS

GLU

SER

5

TYR

SER

ILE

TYR

VAL

TYR

LYS

VAL

LEU

LYS

6

GLN

VAL

HIS

PRO

ASP

THR

GLY

ILE

SER

7

LYS

ALA

MET

GLY

ILE

MET

ASN

SER

PHE

VAL

8

ASN

ASP

ILE

PHE

GLU

ARG

ILE

ALA

GLY

GLU

9

ALA

SER

ARG

LEU

ALA

HIS

TYR

ASN

LYS

ARG

10

SER

THR

ILE

THR

SER

ARG

GLU

ILE

GLN

THR

11

ALA

VAL

ARG

LEU

PRO

GLY

GLU

LEU

12

ALA

LYS

HIS

ALA

VAL

SER

GLU

GLY

THR

LYS

13

ALA

VAL

THR

LYS

TYR

THR

SER

ALA

LYS

Samples:

sample_1: entity_1, [U-13C; U-15N; U-2H], 0.1 – 0.3 mM; entity_2, [U-13C; U-15N; U-2H], 0.1 – 0.3 mM; MES 25 mM; KCl 400 mM; H2O 90%; D2O, [U-99% 2H], 10%

sample_conditions_1: ionic strength: 400 mM; pH: 6.0; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
TROSY-HN(CO)CACB	sample_1	isotropic	sample_conditions_1
TROSY-HNCACB	sample_1	isotropic	sample_conditions_1
TROSY-HN(CA)CO	sample_1	isotropic	sample_conditions_1
TROSY-HNCO	sample_1	isotropic	sample_conditions_1
HCCCONH	sample_1	isotropic	sample_conditions_1
TROSY-1H-15N HSQC	sample_1	isotropic	sample_conditions_1
TROSY-1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

CS-Rosetta, Shen, Vernon, Baker and Bax - geometry optimization

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks