BMRB Entry 11597
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PDB ID: 2rvf
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11597
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Shigeno-Nakazawa, Yoko; Kasai, Takuma; Kostyanovskaya, Elina; Pawlak, Jana; Yamagishi, Junya; Okimoto, Noriaki; Taiji, Makoto; Okada, Mariko; Sauka-Spengler, Tatjana; Westbrook, Jody; Satta, Yoko; Kigawa, Takanori; Imamoto, Akira. "A premetazoan origin of the CRK gene family and co-opted signaling network" Sci. Rep. ., .-..
Assembly members:
crka1_SH2_domain, polymer, 104 residues, 11482.751 Da.
Natural source: Common Name: Monosiga brevicollis Taxonomy ID: 81824 Superkingdom: Eukaryota Kingdom: not available Genus/species: Monosiga brevicollis
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P100927-01
Entity Sequences (FASTA):
crka1_SH2_domain: GSSGSSGMAEAAAPWYHGPL
SRTDAENSLLRMPEGTFLVR
DSTSSPGDYVLSCSENGKVT
HYKLSAEEGKIRIDTHLFDN
LDAAITFYMEHELEYSSLKQ
PLQR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 440 |
| 15N chemical shifts | 101 |
| 1H chemical shifts | 685 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Monosiga brevicollis CRK/CRKL homolog (crka1) SH2 domain | 1 |
Entities:
Entity 1, Monosiga brevicollis CRK/CRKL homolog (crka1) SH2 domain 104 residues - 11482.751 Da.
Residues -6 to 0 are cloning artifacts. GenBank entry KT795324 which corresponds to mRNA sequence of Monosiga brevicollis crka1 is not released yet. The nucleotide sequence of KT795324 is: TGCGCCTCGATCATTTTGAGCCCCAGGGCCTTTGTTGTGCATCATGGCGGAAGCCGCAGCACCCTGGTATCATGGGCCGC TGAGCCGCACCGATGCAGAAAACAGTCTCTTGCGTATGCCCGAAGGGACTTTCTTGGTCCGCGACAGCACCTCGTCCCCC GGAGATTATGTCCTCTCATGCAGCGAGAATGGCAAGGTGACGCACTACAAGCTCTCGGCCGAAGAGGGCAAGATTCGTAT CGATACACACCTCTTTGACAACCTGGATGCTGCCATCACCTTTTACATGGAGCACGAGTTGGAATACAGTTCGCTCAAAC AGCCCCTGCAACGGCAATCATCATGGCGCGATGACGAGCTTGTCGCCACGGTCATGTTCTCTGCAGAAGGGCTGTACAAG TTTGTCGGCCGCAATGAAAACGATTTACCCTTTGACAAGGGGGAGCTTCTCAATGTCTTGCAGCTAACACAGGACAATTG GTGGATGGCCCAGAGTCAGAAAACGCTCAAGATTGGCTACGTTCCCGTCAATTATGTCCGGGCGCTAGAGCAGCCAGCTG CCCCGCCACCGCGCCCTGCTCCACGTCCTGCCGAGGAGGAGTCTTCCAGGCTTGAGCGAACCGAATCCCGTCGTACGCCG GCCAGCCACCATCGACCCAAGGAACCCATGCCGGCAGGTGCCGTCATGACACGAGCTCCGGCTCGACATCACCAAGAGGC AAAGGCACCCCCTGCACGGCCTCAGCCGACTGGGCCTTCTGCCGCGGGTCCGATAGCCGCTGCTGCTCCGCCTCCACGTC CCAAAACATCAAAGCCCACGACCGACTTTAGTGCGCTCAAGGCTCAACTGCAGGAGCCAATTGTTTCCAATCCCACGCCC ACCTCGACGGCCCCAGAGCAGGCGGCTTTGCCCGCCGAAACCACGGCAGAAGTCTCAACACCAACGCAGCCGCTCGCATC TGCTCCGCCCACTTCTACGGCCTCGCATGAAGCGCCGGCAAGCTCTGCCGCACCCGCTACTCAGCACGGCGCAGGGTCAG AGGATCACGCTACCGCTCCCGACTCGGAGCCTCCCATCACTTTGGTCCGCGCCATCACCGCCATCATCCCCAACGCCTAT GACAATGATGCCTTGGTCTTGGAGGAAAATGATTTGGTTCACGTGCTGGATGGCGGCGAAGGCGCACTGGAGCGGGGCCG CGTTCTCAGAACTGGACAGATCGGCTTGTATCCCTTTGCAAAGACTGAGGATATTGAGAGCCACAACTACGATGCCGAAG TGAAGGCAGCCGTCGCTGACTACCTGAAGCGGTTTCCAGATGCATGCTAGCAAACCATAGGTGGCTATTGGCGACGGTCG TGAGATACGT And the translated protein sequence is: MAEAAAPWYHGPLSRTDAENSLLRMPEGTFLVRDSTSSPGDYVLSCSENGKVTHYKLSAEEGKIRIDTHLFDNLDAAITF YMEHELEYSSLKQPLQRQSSWRDDELVATVMFSAEGLYKFVGRNENDLPFDKGELLNVLQLTQDNWWMAQSQKTLKIGYV PVNYVRALEQPAAPPPRPAPRPAEEESSRLERTESRRTPASHHRPKEPMPAGAVMTRAPARHHQEAKAPPARPQPTGPSA AGPIAAAAPPPRPKTSKPTTDFSALKAQLQEPIVSNPTPTSTAPEQAALPAETTAEVSTPTQPLASAPPTSTASHEAPAS SAAPATQHGAGSEDHATAPDSEPPITLVRAITAIIPNAYDNDALVLEENDLVHVLDGGEGALERGRVLRTGQIGLYPFAK TEDIESHNYDAEVKAAVADYLKRFPDAC
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | MET | ALA | GLU | ||||
| 2 | ALA | ALA | ALA | PRO | TRP | TYR | HIS | GLY | PRO | LEU | ||||
| 3 | SER | ARG | THR | ASP | ALA | GLU | ASN | SER | LEU | LEU | ||||
| 4 | ARG | MET | PRO | GLU | GLY | THR | PHE | LEU | VAL | ARG | ||||
| 5 | ASP | SER | THR | SER | SER | PRO | GLY | ASP | TYR | VAL | ||||
| 6 | LEU | SER | CYS | SER | GLU | ASN | GLY | LYS | VAL | THR | ||||
| 7 | HIS | TYR | LYS | LEU | SER | ALA | GLU | GLU | GLY | LYS | ||||
| 8 | ILE | ARG | ILE | ASP | THR | HIS | LEU | PHE | ASP | ASN | ||||
| 9 | LEU | ASP | ALA | ALA | ILE | THR | PHE | TYR | MET | GLU | ||||
| 10 | HIS | GLU | LEU | GLU | TYR | SER | SER | LEU | LYS | GLN | ||||
| 11 | PRO | LEU | GLN | ARG |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks