BMRB Entry 11590

Click here to enlarge.

PDB ID: 2rv8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11590
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solution Structure of the PhoP DNA-Binding Domain from Mycobacterium tuberculosis
Deposition date:
2015-04-17
Original release date:
2015-08-10
Authors:
Macdonald, Ramsay; Sarkar, Dibyendu; Amer, Brendan; Clubb, Robert
Citation:

Citation: Macdonald, Ramsay; Sarkar, Dibyendu; Amer, Brendan; Clubb, Robert. "Solution structure of the PhoP DNA-binding domain from Mycobacterium tuberculosis"  J. Biomol. NMR ., .-. (2015).
PubMed: 26209027

Assembly members:

Assembly members:
PhoPC, polymer, 128 residues, 14723.858 Da.

Natural source:

Natural source:   Common Name: Mycobacterium tuberculosis   Taxonomy ID: 1773   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PhoPC: MGSSHHHHHHSSGLVPRGSH MKGNKEPRNVRLTFADIELD EETHEVWKAGQPVSLSPTEF TLLRYFVINAGTVLSKPKIL DHVWRYDFGGDVNVVESYVS YLRRKIDTGEKRLLHTLRGV GYVLREPR

Data sets:
Data typeCount
13C chemical shifts453
15N chemical shifts98
1H chemical shifts673

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PhoP DNA-Binding Domain1

Entities:

Entity 1, PhoP DNA-Binding Domain 128 residues - 14723.858 Da.

Residues 120-141 represent a histidine-tag

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METLYSGLYASNLYSGLUPROARGASNVAL
4   ARGLEUTHRPHEALAASPILEGLULEUASP
5   GLUGLUTHRHISGLUVALTRPLYSALAGLY
6   GLNPROVALSERLEUSERPROTHRGLUPHE
7   THRLEULEUARGTYRPHEVALILEASNALA
8   GLYTHRVALLEUSERLYSPROLYSILELEU
9   ASPHISVALTRPARGTYRASPPHEGLYGLY
10   ASPVALASNVALVALGLUSERTYRVALSER
11   TYRLEUARGARGLYSILEASPTHRGLYGLU
12   LYSARGLEULEUHISTHRLEUARGGLYVAL
13   GLYTYRVALLEUARGGLUPROARG

Related Database Links:

PDB 2RV8 3R0J
DBJ BAH25072 BAL64650 BAQ04662 GAA44527
EMBL CAL70795 CCC25841 CCC43101 CCC63367 CCE36300
GB AAK45023 ABQ72495 ABR05115 ACT23809 AEB02905
REF NP_215271 NP_854438 WP_003403867 WP_003915456 WP_012054162

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks