BMRB Entry 11546

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PDB ID: 2rvk
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11546
MolProbity Validation Chart

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Title:
1H, 15N and 13C resonance assignments of the conserved domain in the middle of Schizosaccharomyces pombe SAPK-interacting protein 1
Deposition date:
2013-11-27
Original release date:
2015-07-27
Authors:
Kataoka, Saori; Furuita, Kyoko; Hattori, Yoshikazu; Kobayashi, Naohiro; Ikegami, Takahisa; Shiozaki, Kazuhiro; Fujiwara, Toshimichi; Kojima, Chojiro
Citation:

Citation: Furuita, Kyoko; Kataoka, Saori; Hattori, Yoshikazu; Kobayashi, Naohiro; Ikegami, Takahisa; Shiozaki, Kazuhiro; Fujiwara, Toshimichi; Kojima, Chojiro. "Utilization of paramagnetic relaxation enhancements for high-resolution NMR structure determination of a soluble loop-rich protein with sparse NOE distance restraints"  Biomol. NMR Assignments 61, 55-64 (2015).
PubMed: 25428765

Assembly members:

Assembly members:
sin1, polymer, 160 residues, 17037.0 Da.

Natural source:

Natural source:   Common Name: fission yeast   Taxonomy ID: 4896   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Schizosaccharomyces pombe

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pCold-GST

Entity Sequences (FASTA):

Entity Sequences (FASTA):
sin1: GPGHMGSVSNAKAPTSALRA LLEHKENSSQNGPLAENFAT FSGHAESNALRLNIYFPSSE SPSKPLFVELRKNVLVSEAI GYILLQYVNQQLVPPIEDEA QNPNYWNLRIVEDDGELDED FPALDRVGPLSKFGFDAFAL VKATPAQIKENQAAYPFKSK

Data sets:
Data typeCount
13C chemical shifts559
15N chemical shifts144
1H chemical shifts890

Additional metadata:

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  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1sin11

Entities:

Entity 1, sin1 160 residues - 17037.0 Da.

1   GLYPROGLYHISMETGLYSERVALSERASN
2   ALALYSALAPROTHRSERALALEUARGALA
3   LEULEUGLUHISLYSGLUASNSERSERGLN
4   ASNGLYPROLEUALAGLUASNPHEALATHR
5   PHESERGLYHISALAGLUSERASNALALEU
6   ARGLEUASNILETYRPHEPROSERSERGLU
7   SERPROSERLYSPROLEUPHEVALGLULEU
8   ARGLYSASNVALLEUVALSERGLUALAILE
9   GLYTYRILELEULEUGLNTYRVALASNGLN
10   GLNLEUVALPROPROILEGLUASPGLUALA
11   GLNASNPROASNTYRTRPASNLEUARGILE
12   VALGLUASPASPGLYGLULEUASPGLUASP
13   PHEPROALALEUASPARGVALGLYPROLEU
14   SERLYSPHEGLYPHEASPALAPHEALALEU
15   VALLYSALATHRPROALAGLNILELYSGLU
16   ASNGLNALAALATYRPROPHELYSSERLYS

Related Database Links:

EMBL CAB66311
GB AAD37449
PIR T51293
REF NP_594703
SP Q9P7Y9
AlphaFold Q9P7Y9

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