BMRB Entry 11544

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PDB ID: 2ru4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11544
MolProbity Validation Chart

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Title:
DESIGNED ARMADILLO REPEAT PROTEIN SELF-ASSEMBLED COMPLEX (YIIM2-MAII)
Deposition date:
2013-11-22
Original release date:
2014-07-21
Authors:
Zerbe, Oliver; Christen, Martin; Plueckthun, Andreas; Watson, Randall
Citation:

Citation: Watson, Randall; Christen, Martin; Ewald, Christina; Bumback, Fabian; Reichen, Christian; Mihajlovic, Maja; Schmidt, E.; Guntert, Peter; Caflisch, Amedeo; Plueckthun, Andreas; Zerbe, Oliver. "Spontaneous Self-Assembly of Fragments of Engineered Armadillo Repeat Proteins into Folded Proteins"  Structure 22, 985-995 (2014).
PubMed: 24931467

Assembly members:

Assembly members:
YM2, polymer, 115 residues, 12193.641 Da.
MA, polymer, 84 residues, 9104.170 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pLIC_CR

Entity Sequences (FASTA):

Entity Sequences (FASTA):
YM2: GELPQMTQQLNSDDMQEQLS ATRKFSQILSDGNEQIQAVI DAGALPALVQLLSSPNEQIL QEALWALSNIASGGNEQIQA VIDAGALPALVQLLSSPNEQ ILQEALWALSNIASG
MA: GNEQIQAVIDAGALPALVQL LSSPNEQILQEALWALSNIA SGGNEQKQAVKEAGALEKLE QLQSHENEKIQKEAQEALEK LQSH

Data sets:
Data typeCount
13C chemical shifts632
15N chemical shifts186
1H chemical shifts1081

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1YM21
2MA2

Entities:

Entity 1, YM2 115 residues - 12193.641 Da.

1   GLYGLULEUPROGLNMETTHRGLNGLNLEU
2   ASNSERASPASPMETGLNGLUGLNLEUSER
3   ALATHRARGLYSPHESERGLNILELEUSER
4   ASPGLYASNGLUGLNILEGLNALAVALILE
5   ASPALAGLYALALEUPROALALEUVALGLN
6   LEULEUSERSERPROASNGLUGLNILELEU
7   GLNGLUALALEUTRPALALEUSERASNILE
8   ALASERGLYGLYASNGLUGLNILEGLNALA
9   VALILEASPALAGLYALALEUPROALALEU
10   VALGLNLEULEUSERSERPROASNGLUGLN
11   ILELEUGLNGLUALALEUTRPALALEUSER
12   ASNILEALASERGLY

Entity 2, MA 84 residues - 9104.170 Da.

1   GLYASNGLUGLNILEGLNALAVALILEASP
2   ALAGLYALALEUPROALALEUVALGLNLEU
3   LEUSERSERPROASNGLUGLNILELEUGLN
4   GLUALALEUTRPALALEUSERASNILEALA
5   SERGLYGLYASNGLUGLNLYSGLNALAVAL
6   LYSGLUALAGLYALALEUGLULYSLEUGLU
7   GLNLEUGLNSERHISGLUASNGLULYSILE
8   GLNLYSGLUALAGLNGLUALALEUGLULYS
9   LEUGLNSERHIS

Related Database Links:

PDB 2RU4 2RU5
BMRB 11548

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