BMRB Entry 11531

Assembly:

Entities:

Entity 1, Chi18aC_CBD 105 residues - 10609.385 Da.

Samples:

sample_1: Chi18aC_CBD, [U-15N], 1.1 mM; potassium phosphate 20 mM; sodium azide 0.02%; DTT 1 mM; H2O 90%; D2O, [U-2H], 10%

sample_2: Chi18aC_CBD, [U-13C; U-15N], 0.64 mM; potassium phosphate 20 mM; sodium azide 0.02%; DTT 1 mM; H2O 90%; D2O, [U-2H], 10%

sample_3: Chi18aC_CBD, [U-13C; U-15N], 0.71 mM; potassium phosphate 20 mM; sodium azide 0.02%; DTT 1 mM; D2O, [U-2H], 100%

sample_4: Chi18aC_CBD, [U-15% 13C; U-15N], 0.37 mM; potassium phosphate 20 mM; sodium azide 0.02%; DTT 1 mM; H2O 90%; D2O, [U-2H], 10%

sample_5: Chi18aC_CBD 1.0 mM; potassium phosphate 20 mM; sodium azide 0.02%; DTT 1 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 303 K

Experiments:

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

TALOS, Cornilescu, Delaglio and Bax - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

• Bruker DRX 500 MHz
• Bruker DRX 600 MHz
• Bruker DRX 800 MHz
• Bruker Avance 400 MHz