BMRB Entry 11525

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for PriC N-terminal domain
Published: 2013-08-05

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PDB ID: 2rt6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11525
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for PriC N-terminal domain

Deposition date:

2013-04-24

Original release date:

2013-08-05

Authors:

Aramaki, Takahiko; Abe, Yoshito; Katayama, Tsutomu; Ueda, Tadashi

Citation:

Citation: Aramaki, Takahiko; Abe, Yoshito; Katayama, Tsutomu; Ueda, Tadashi. "Solution structure of the N-terminal domain of a replication restart primosome factor, PriC, in Escherichia coli" Protein Sci. ., .-..
PubMed: 23868391

Assembly members:

Assembly members:
PriC_N-terminal_domain, polymer, 98 residues, 11007.640 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PriC_N-terminal_domain: MKTALLLEKLEGQLATLRQR CAPVSQFATLSARFDRHLFQ TRATTLQACLDEAGDNLAAL RHAVEQQQLPQVAWLAEHLA AQLEAIAREASAWSLREW

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	423
15N chemical shifts	112
1H chemical shifts	696

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PriC_N-terminal_domain	1

Entities:

Entity 1, PriC_N-terminal_domain 98 residues - 11007.640 Da.

1

MET

LYS

THR

ALA

LEU

GLU

LYS

LEU

2

GLU

GLY

GLN

LEU

ALA

THR

LEU

ARG

GLN

ARG

3

CYS

ALA

PRO

VAL

SER

GLN

PHE

ALA

THR

LEU

4

SER

ALA

ARG

PHE

ASP

ARG

HIS

LEU

PHE

GLN

5

THR

ARG

ALA

THR

LEU

GLN

ALA

CYS

LEU

6

ASP

GLU

ALA

GLY

ASP

ASN

LEU

ALA

LEU

7

ARG

HIS

ALA

VAL

GLU

GLN

LEU

PRO

8

GLN

VAL

ALA

TRP

LEU

ALA

GLU

HIS

LEU

ALA

9

ALA

GLN

LEU

GLU

ALA

ILE

ALA

ARG

GLU

ALA

10

SER

ALA

TRP

SER

LEU

ARG

GLU

TRP

Samples:

sample_1: PriC N-terminal domain, [U-99% 13C; U-99% 15N], 0.5 mM; sodium chloride 150 mM; H2O 90%; D2O 10%

sample_2: PriC N-terminal domain, [U-98% 15N], 0.2 mM; sodium chloride 150 mM; D2O 100%

sample_conditions_1: ionic strength: 150 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

Olivia v1.16.6, Olivia Developer Team - chemical shift assignment, data analysis

CNS v1.21, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

NMR spectrometers:

Varian INOVA 600 MHz

PDB	2RT6
DBJ	BAA03055 BAB33943 BAE76246 BAG76016 BAI23841
EMBL	CAP75000 CAQ30940 CAQ97342 CAR01811 CAR06700
GB	AAB40221 AAC73569 AAN42067 AAN79064 AAP15944
REF	NP_308547 NP_415000 NP_706360 WP_000626986 WP_000844845
SP	P23862
AlphaFold	P23862