BMRB Entry 11496
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PDB ID: 2rsn
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11496
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Ishida, Mayumi; Shimojo, Hideaki; Hayashi, Aki; Kawaguchi, Rika; Ohtani, Yasuko; Uegaki, Koichi; Nishimura, Yoshifumi; Nakayama, Jun-ichi. "Intrinsic nucleic Acid-binding activity of chp1 chromodomain is required for heterochromatic gene silencing" Mol. Cell 47, 228-241 (2012).
PubMed: 22727667
Assembly members:
Chp1_chromodomain, polymer, 75 residues, 8909.997 Da.
Histone_H3K9me3, polymer, 18 residues, 2025.379 Da.
Natural source: Common Name: fission yeast Taxonomy ID: 4896 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Schizosaccharomyces pombe
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pCOLD
Entity Sequences (FASTA):
Chp1_chromodomain: MVSVKPLPDIDSNEGETDAD
VYEVEDILADRVNKNGINEY
YIKWAGYDWYDNTWEPEQNL
FGAEKVLKKWKKRKK
Histone_H3K9me3: ARTKQTARXSTGGKAPRY
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 352 |
| 15N chemical shifts | 82 |
| 1H chemical shifts | 599 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | entity_2 | 2 |
Entities:
Entity 1, entity_1 75 residues - 8909.997 Da.
| 1 | MET | VAL | SER | VAL | LYS | PRO | LEU | PRO | ASP | ILE | ||||
| 2 | ASP | SER | ASN | GLU | GLY | GLU | THR | ASP | ALA | ASP | ||||
| 3 | VAL | TYR | GLU | VAL | GLU | ASP | ILE | LEU | ALA | ASP | ||||
| 4 | ARG | VAL | ASN | LYS | ASN | GLY | ILE | ASN | GLU | TYR | ||||
| 5 | TYR | ILE | LYS | TRP | ALA | GLY | TYR | ASP | TRP | TYR | ||||
| 6 | ASP | ASN | THR | TRP | GLU | PRO | GLU | GLN | ASN | LEU | ||||
| 7 | PHE | GLY | ALA | GLU | LYS | VAL | LEU | LYS | LYS | TRP | ||||
| 8 | LYS | LYS | ARG | LYS | LYS |
Entity 2, entity_2 18 residues - 2025.379 Da.
ARTKQTAR(M3L)STGGKAPRY
| 1 | ALA | ARG | THR | LYS | GLN | THR | ALA | ARG | M3L | SER | ||||
| 2 | THR | GLY | GLY | LYS | ALA | PRO | ARG | TYR |
Related Database Links:
| PDB | 2RSN 3G7L 3N9O 4FT4 4GY5 4LKA |
| EMBL | CAA92382 |
| REF | NP_593666 |
| SP | Q10103 |
| GB | AAY98280 |
| AlphaFold | Q10103 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated peaks