BMRB Entry 11469

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PDB ID: 2rsd
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11469
MolProbity Validation Chart

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Title:
Solution structure of the plant homeodomain (PHD) of the E3 SUMO ligase Siz1 from rice
Deposition date:
2012-01-11
Original release date:
2012-08-13
Authors:
Shindo, Heisaburo; Tsuchiya, Wataru; Suzuki, Rintaro; Yamazaki, Toshimasa
Citation:

Citation: Shindo, Heisaburo; Suzuki, Rintaro; Tsuchiya, Wataru; Taichi, Misako; Nishiuchi, Yuji; Yamazaki, Toshimasa. "PHD finger of the SUMO ligase Siz/PIAS family in rice reveals specific binding for methylated histone H3 at lysine 4 and arginine 2"  FEBS Lett. 586, 1783-1789 (2012).
PubMed: 22626555

Assembly members:

Assembly members:
entity_1, polymer, 68 residues, 7671.758 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Oryza sativa subsp. japonica   Taxonomy ID: 39947   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Oryza Oryza sativa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T-3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSDSFQPEAKVRCICSSTMV NDSMIQCEDQRCQVWQHLNC VLIPDKPGESAEVPPVFYCE LCRLSRAD

Data sets:
Data typeCount
13C chemical shifts308
15N chemical shifts72
1H chemical shifts460

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2ZINC ION_12
3ZINC ION_22

Entities:

Entity 1, entity_1 68 residues - 7671.758 Da.

Residues 105-106 represent a non-native sequence from affinity tag.

1   GLYSERASPSERPHEGLNPROGLUALALYS
2   VALARGCYSILECYSSERSERTHRMETVAL
3   ASNASPSERMETILEGLNCYSGLUASPGLN
4   ARGCYSGLNVALTRPGLNHISLEUASNCYS
5   VALLEUILEPROASPLYSPROGLYGLUSER
6   ALAGLUVALPROPROVALPHETYRCYSGLU
7   LEUCYSARGLEUSERARGALAASP

Entity 2, ZINC ION_1 - Zn - 65.409 Da.

1   ZN

Related Database Links:

PDB 2RSD
DBJ BAF16431 BAG97182 BAS92058
GB AAT39226 EEC78434 EEE62174
REF NP_001054517
SP Q6L4L4
AlphaFold Q6L4L4

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