BMRB Entry 11463

Name: Solution structure of the bromodomain of human BRPF1 in complex with histone H4K5ac peptide
Published: 2013-03-26

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PDB ID: 2rs9
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11463
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the bromodomain of human BRPF1 in complex with histone H4K5ac peptide

Deposition date:

2011-12-08

Original release date:

2013-03-26

Authors:

Qin, X. R.; Nagashima, T.; Umehara, T.; Hayashi, F.; Yokoyama, S.

Citation:

Citation: Qin, X. R.; Hayashi, F.; Yokoyama, S.. "Site-specific histone recognition by the bromodomain of Brpf1 and the role in MOZ/MORF histone acetyltransferase complexes" .

Assembly members:

Assembly members:
An_acetylated-lysine_5_of_histone_4_peptide, polymer, 10 residues, 960.099 Da.
Bromodomain, polymer, 121 residues, 13777.645 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis Host organism: E. coli - cell free

Entity Sequences (FASTA):

Entity Sequences (FASTA):
An_acetylated-lysine_5_of_histone_4_peptide: SGRGXGGKGL
Bromodomain: GSSGSSGFLILLRKTLEQLQ EKDTGNIFSEPVPLSEVPDY LDHIKKPMDFFTMKQNLEAY RYLNFDDFEEDFNLIVSNCL KYNAKDTIFYRAAVRLREQG GAVLRQARRQAEKMGSGPSS G

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	550
15N chemical shifts	130
1H chemical shifts	919

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	An_acetylated-lysine_5_of_histone_4_peptide	1
2	Bromodomain	2

Entities:

Entity 1, An_acetylated-lysine_5_of_histone_4_peptide 10 residues - 960.099 Da.

SGRG(ALY)GGKGL acetylated-lysine 5

1

SER

GLY

ARG

GLY

ALY

GLY

LYS

GLY

LEU

Entity 2, Bromodomain 121 residues - 13777.645 Da.

41-47 and 156-161 represent a non-native affinity tag

1

GLY

SER

GLY

SER

GLY

PHE

LEU

ILE

2

LEU

ARG

LYS

THR

LEU

GLU

GLN

LEU

GLN

3

GLU

LYS

ASP

THR

GLY

ASN

ILE

PHE

SER

GLU

4

PRO

VAL

PRO

LEU

SER

GLU

VAL

PRO

ASP

TYR

5

LEU

ASP

HIS

ILE

LYS

PRO

MET

ASP

PHE

6

PHE

THR

MET

LYS

GLN

ASN

LEU

GLU

ALA

TYR

7

ARG

TYR

LEU

ASN

PHE

ASP

PHE

GLU

8

ASP

PHE

ASN

LEU

ILE

VAL

SER

ASN

CYS

LEU

9

LYS

TYR

ASN

ALA

LYS

ASP

THR

ILE

PHE

TYR

10

ARG

ALA

VAL

ARG

LEU

ARG

GLU

GLN

GLY

11

GLY

ALA

VAL

LEU

ARG

GLN

ALA

ARG

GLN

12

ALA

GLU

LYS

MET

GLY

SER

GLY

PRO

SER

13

GLY

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
13C-edited (F1)	sample_1	isotropic	sample_conditions_1
13C/15N-filtered (F3) 3D NOESY	sample_1	isotropic	sample_conditions_1

EMBL	P62805 CAD28495
PDB	2D9E 2RS9 4LC2 4UYE
DBJ	BAC31528 BAE33691 BAG10583 BAG57257
GB	AAB02119 AAF19605 AAH05647 AAH46521 AAI38362
REF	NP_001178501 NP_001269055 NP_001269056 NP_004625 NP_084454
SP	P55201
TPG	DAA17219
AlphaFold	P55201

BMRB Entry 11463

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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