BMRB Entry 11439

Title:
NMR Structure of LC4 transmembrane segment of CCR5
Deposition date:
2011-04-11
Original release date:
2013-03-20
Authors:
Miyamoto, Kazuhide
Citation:

Citation: Miyamoto, Kazuhide; Togiya, Kayo. "Solution Structure of LC4 Transmembrane Segment of CCR5"  PLoS ONE 6, e20452-e20452 (2011).
PubMed: 21647380

Assembly members:

Assembly members:
LC4, polymer, 18 residues, 1965.317 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LC4: VFASLPGIIFTRSQKEGL

Data sets:
Data typeCount
1H chemical shifts19

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LC41

Entities:

Entity 1, LC4 18 residues - 1965.317 Da.

1   VALPHEALASERLEUPROGLYILEILEPHE
2   THRARGSERGLNLYSGLUGLYLEU

Samples:

sample_1: LC4 2 mM; phosphate 80 mM; sodium dodecyl-d25 sulfate (SDS) 200 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 280 mM; pH: 4.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - refinement

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

PDB
DBJ BAF84821 BAG73996
EMBL CAA62796 CAA67767
GB AAB09551 AAB17071 AAB57793 AAB58446 AAB62553
REF NP_000570 NP_001009046 NP_001093638 NP_001128969 NP_001244145
SP O97881 O97883 P51681 P56439 P56440
AlphaFold O97881 O97883 P51681 P56439 P56440