BMRB Entry 11439
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11439
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Citation: Miyamoto, Kazuhide; Togiya, Kayo. "Solution Structure of LC4 Transmembrane Segment of CCR5" PLoS ONE 6, e20452-e20452 (2011).
PubMed: 21647380
Assembly members:
LC4, polymer, 18 residues, 1965.317 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
LC4: VFASLPGIIFTRSQKEGL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 19 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | LC4 | 1 |
Entities:
Entity 1, LC4 18 residues - 1965.317 Da.
| 1 | VAL | PHE | ALA | SER | LEU | PRO | GLY | ILE | ILE | PHE | ||||
| 2 | THR | ARG | SER | GLN | LYS | GLU | GLY | LEU |
Samples:
sample_1: LC4 2 mM; phosphate 80 mM; sodium dodecyl-d25 sulfate (SDS) 200 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 280 mM; pH: 4.5; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - refinement
NMR spectrometers:
- Bruker Avance 500 MHz