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PDB ID: 1x4s
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11363
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of zinc finger HIT domain in protein FON

Deposition date:

2010-09-07

Original release date:

2011-09-07

Authors:

He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.

Citation:

Citation: He, Fahu; Umehara, Takashi; Tsuda, Kengo; Inoue, Makoto; Kigawa, Takanori; Matsuda, Takayoshi; Yabuki, Takashi; Aoki, Masaaki; Seki, Eiko; Terada, Takaho; Shirouzu, Mikako; Tanaka, Akiko; Sugano, Sumio; Muto, Yutaka; Yokoyama, Shigeyuki. "Solution structure of the zinc finger HIT domain in protein FON. " Protein Sci 16, 1577-1587 (2007).
PubMed: 17656577

Assembly members:

Assembly members:
Zinc finger HIT domain, polymer, 59 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040906-13

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Zinc finger HIT domain: GSSGSSGMEPAGPCGFCPAG EVQPARYTCPRCNAPYCSLR CYRTHGTCAENFYSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	211
15N chemical shifts	46
1H chemical shifts	304

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Zinc finger HIT domain	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, Zinc finger HIT domain 59 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

GLU

PRO

2

ALA

GLY

PRO

CYS

GLY

PHE

CYS

PRO

ALA

GLY

3

GLU

VAL

GLN

PRO

ALA

ARG

TYR

THR

CYS

PRO

4

ARG

CYS

ASN

ALA

PRO

TYR

CYS

SER

LEU

ARG

5

CYS

TYR

ARG

THR

HIS

GLY

THR

CYS

ALA

GLU

6

ASN

PHE

TYR

SER

GLY

PRO

SER

GLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: Zinc finger HIT domain, [U-13C; U-15N], 0.8 mM; d-Tris-HCl 20 mM; NaCl 100 mM; ZnCl2 100 uM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio F. - processing

NMRView v5.0.4, Johnson B.A. - data analysis

Kujira v0.863, Kobayashi N. - data analysis

CYANA v2.0.17, Guntert P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 11363