BMRB Entry 11360

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PDB ID: 2e61
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11360
MolProbity Validation Chart

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Title:
Assigned chemical shifts of the zf-CW domain with H3 trimethyl K4 peptide
Deposition date:
2010-09-07
Original release date:
2011-01-04
Authors:
He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.
Citation:

Citation: He, Fahu; Umehara, Takashi; Saito, Kohei; Harada, Takushi; Watanabe, Satoru; Yabuki, Takashi; Kigawa, Takanori; Takahashi, Mari; Kuwasako, Kanako; Tsuda, Kengo; Matsuda, Takayoshi; Aoki, Masaaki; Seki, Eiko; Kobayashi, Naohiro; Guntert, Peter; Yokoyama, Shigeyuki; Muto, Yutaka. "Structural insight into the zinc finger CW domain as a histone modification reader."  Structure 18, 1127-1139 (2010).
PubMed: 20826339

Assembly members:

Assembly members:
zf-CW domain, polymer, 69 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
H3 trimethyl K4 peptide, polymer, 20 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P060116-12

Entity Sequences (FASTA):

Entity Sequences (FASTA):
zf-CW domain: GSSGSSGEISGFGQCLVWVQ CSFPNCGKWRRLCGNIDPSV LPDNWSCDQNTDVQYNRCDI PEETWTGLE
H3 trimethyl K4 peptide: ARTXQTARKSTGGKAPRKQL

Data sets:
Data typeCount
15N chemical shifts57
1H chemical shifts57

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1zf-CW domain1
2ZINC ION2
3H3 trimethyl K4 peptide3

Entities:

Entity 1, zf-CW domain 69 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLUILESER
2   GLYPHEGLYGLNCYSLEUVALTRPVALGLN
3   CYSSERPHEPROASNCYSGLYLYSTRPARG
4   ARGLEUCYSGLYASNILEASPPROSERVAL
5   LEUPROASPASNTRPSERCYSASPGLNASN
6   THRASPVALGLNTYRASNARGCYSASPILE
7   PROGLUGLUTHRTRPTHRGLYLEUGLU

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Entity 3, H3 trimethyl K4 peptide 20 residues - Formula weight is not available

1   ALAARGTHRM3LGLNTHRALAARGLYSSER
2   THRGLYGLYLYSALAPROARGLYSGLNLEU

Related Database Links:

BMRB 11115 11358 11359 11361 11362 11359
PDB 2E61 2RR4 1PU9 2UXN 3N9P 4MZG
DBJ BAA91424 BAC04028 BAG62414 BAG63489
EMBL CAB66669 CAT02683 CAT02685 CAT02686 CAT02687 CAT02689
GB AAH02725 AIC60275 EAW76538 EAW76539 EAW76540 AAT28135 AAT28136 AAT28137 AAT28138 AAT28139
REF NP_001244937 NP_060454 XP_002803268 XP_003318693 XP_003318694 XP_001194483 XP_002028918 XP_002904927 XP_002927153 XP_003502247
SP Q9H0M4
AlphaFold Q9H0M4

Download HSQC peak lists in one of the following formats:
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