BMRB Entry 11357

Title:
Solution structure of WW domain in transcription elongation regulator 1
Deposition date:
2010-09-07
Original release date:
2011-09-07
Authors:
He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.
Citation:

Citation: He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.. "Solution structure of WW domain in transcription elongation regulator 1"  .

Assembly members:

Assembly members:
WW domain, polymer, 73 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050822-13

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts295
15N chemical shifts61
1H chemical shifts454

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1WW domain1

Entities:

Entity 1, WW domain 73 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLYSALALYS
2   PROVALALATHRALAPROILEPROGLYTHR
3   PROTRPCYSVALVALTRPTHRGLYASPGLU
4   ARGVALPHEPHETYRASNPROTHRTHRARG
5   LEUSERMETTRPASPARGPROASPASPLEU
6   ILEGLYARGALAASPVALASPLYSILEILE
7   GLNGLUPROPROHISLYSLYSSERGLYPRO
8   SERSERGLY

Samples:

sample_1: WW domain, [U-13C; U-15N], 0.8 mM; phosphate buffer NA 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio F. - processing

NMRView v5.0.4, Johnson B.A. - data analysis

Kujira v0.9321, Kobayashi N. - data analysis

CYANA v2.0.17, Guntert P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
REF XP_008143734

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks