BMRB Entry 11349

Name: Solution structure of LIM domain in LIM-protein 3
Published: 2011-09-07

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PDB ID: 1x4k
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11349
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of LIM domain in LIM-protein 3

Deposition date:

2010-09-07

Original release date:

2011-09-07

Authors:

He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.

Citation:

Citation: He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.. "Solution structure of LIM domain in LIM-protein 3" .

Assembly members:

Assembly members:
LIM domain, polymer, 72 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040830-05

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LIM domain: GSSGSSGCQECKKTIMPGTR KMEYKGSSWHETCFICHRCQ QPIGTKSFIPKDNQNFCVPC YEKQHASGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	289
15N chemical shifts	66
1H chemical shifts	437

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	LIM domain	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, LIM domain 72 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

CYS

GLN

GLU

2

CYS

LYS

THR

ILE

MET

PRO

GLY

THR

ARG

3

LYS

MET

GLU

TYR

LYS

GLY

SER

TRP

HIS

4

GLU

THR

CYS

PHE

ILE

CYS

HIS

ARG

CYS

GLN

5

GLN

PRO

ILE

GLY

THR

LYS

SER

PHE

ILE

PRO

6

LYS

ASP

ASN

GLN

ASN

PHE

CYS

VAL

PRO

CYS

7

TYR

GLU

LYS

GLN

HIS

ALA

SER

GLY

PRO

SER

8

SER

GLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: LIM domain, [U-13C; U-15N], 0.8 mM; d-Tris-HCl 20 mM; NaCl 100 mM; ZnCl2 100 uM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio F. - processing

NMRView v5.0.4, Johnson B.A. - data analysis

Kujira v0.863, Kobayashi N. - data analysis

CYANA v2.0.17, Guntert P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz