BMRB Entry 11344

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11344
MolProbity Validation Chart

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Title:
Solution structure of the RING domain of the human RING finger protein 141
Deposition date:
2010-08-10
Original release date:
2011-08-19
Authors:
Miyamoto, K.; Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Miyamoto, K.; Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the RING domain of the human RING finger protein 141"  .

Assembly members:

Assembly members:
RING domain, polymer, 70 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P060919-15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RING domain: GSSGSSGRVKQLTDEEECCI CMDGRADLILPCAHSFCQKC IDKWSDRHRNCPICRLQMTG ANESSGPSSG

Data sets:
Data typeCount
13C chemical shifts255
15N chemical shifts61
1H chemical shifts401

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RING domain1
2ZINC ION no.12
3ZINC ION no.22

Entities:

Entity 1, RING domain 70 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYARGVALLYS
2   GLNLEUTHRASPGLUGLUGLUCYSCYSILE
3   CYSMETASPGLYARGALAASPLEUILELEU
4   PROCYSALAHISSERPHECYSGLNLYSCYS
5   ILEASPLYSTRPSERASPARGHISARGASN
6   CYSPROILECYSARGLEUGLNMETTHRGLY
7   ALAASNGLUSERSERGLYPROSERSERGLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: RING domain, [U-13C; U-15N], 1.31 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1.0 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 900 MHz

Related Database Links:

PDB
DBJ BAB24742 BAB27871 BAB29874 BAE32570 BAF82453
EMBL CAH92252
GB AAF30180 AAH18104 AAH18553 AAH88675 AAI09814
REF NP_001001800 NP_001035656 NP_001041484 NP_001088870 NP_001126318
SP Q2XNS1 Q32L15 Q5R7K8 Q6IV57 Q8WVD5
TPG DAA22251
AlphaFold Q2XNS1 Q32L15 Q5R7K8 Q6IV57 Q8WVD5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks