BMRB Entry 11341

Click here to enlarge.

PDB ID: 2ecj
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11341
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structure of the RING domain of the human tripartite motif-containing protein 39

Deposition date:

2010-08-10

Original release date:

2011-08-19

Authors:

Miyamoto, K.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Miyamoto, K.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the RING domain of the human tripartite motif-containing protein 39" .

Assembly members:

Assembly members:
RING domain, polymer, 58 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P060828-17

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RING domain: GSSGSSGALENLQVEASCSV CLEYLKEPVIIECGHNFCKA CITRWWEDLERDFPCPVC

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	234
15N chemical shifts	53
1H chemical shifts	365

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RING domain	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, RING domain 58 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ALA

LEU

GLU

2

ASN

LEU

GLN

VAL

GLU

ALA

SER

CYS

SER

VAL

3

CYS

LEU

GLU

TYR

LEU

LYS

GLU

PRO

VAL

ILE

4

ILE

GLU

CYS

GLY

HIS

ASN

PHE

CYS

LYS

ALA

5

CYS

ILE

THR

ARG

TRP

GLU

ASP

LEU

GLU

6

ARG

ASP

PHE

PRO

CYS

PRO

VAL

CYS

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: RING domain, [U-13C; U-15N], 1.06 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1.0 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2ECJ
DBJ	BAB16374 BAB16375 BAC35409 BAD13703 BAD13704
EMBL	CAE84052 CAH90337
GB	AAH07661 AAH31540 AAH34985 AAP36034 AAP36295
REF	NP_001065263 NP_001121951 NP_001125160 NP_001186048 NP_067076
SP	Q1XHU0 Q6MFZ5 Q9ESN2 Q9HCM9
AlphaFold	Q1XHU0 Q6MFZ5 Q9ESN2 Q9HCM9