BMRB Entry 11338

Name: Solution structure of the RWD domain of human RWD domain containing protein 1
Published: 2011-08-19

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PDB ID: 2ebm
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11338
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the RWD domain of human RWD domain containing protein 1

Deposition date:

2010-08-10

Original release date:

2011-08-19

Authors:

Yoneyama, M.; Tomizawa, T.; Koshiba, S.; Watabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Yoneyama, M.; Tomizawa, T.; Koshiba, S.; Watabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the RWD domain of human RWD domain containing protein 1" .

Assembly members:

Assembly members:
RWD domain, polymer, 128 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P060522-12

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RWD domain: GSSGSSGMTDYGEEQRNELE ALESIYPDSFTVLSENPPSF TITVTSEAGENDETVQTTLK FTYSEKYPDEAPLYEIFSQE NLEDNDVSDILKLLALQAEE NLGMVMIFTLVTAVQEKLNE IVDQIKTR

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	552
15N chemical shifts	130
1H chemical shifts	878

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RWD domain	1

Entities:

Entity 1, RWD domain 128 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

THR

ASP

2

TYR

GLY

GLU

GLN

ARG

ASN

GLU

LEU

GLU

3

ALA

LEU

GLU

SER

ILE

TYR

PRO

ASP

SER

PHE

4

THR

VAL

LEU

SER

GLU

ASN

PRO

SER

PHE

5

THR

ILE

THR

VAL

THR

SER

GLU

ALA

GLY

GLU

6

ASN

ASP

GLU

THR

VAL

GLN

THR

LEU

LYS

7

PHE

THR

TYR

SER

GLU

LYS

TYR

PRO

ASP

GLU

8

ALA

PRO

LEU

TYR

GLU

ILE

PHE

SER

GLN

GLU

9

ASN

LEU

GLU

ASP

ASN

ASP

VAL

SER

ASP

ILE

10

LEU

LYS

LEU

ALA

LEU

GLN

ALA

GLU

11

ASN

LEU

GLY

MET

VAL

MET

ILE

PHE

THR

LEU

12

VAL

THR

ALA

VAL

GLN

GLU

LYS

LEU

ASN

GLU

13

ILE

VAL

ASP

GLN

ILE

LYS

THR

ARG

Samples:

sample_1: RWD domain, [U-13C; U-15N], 1.19 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2EBM
DBJ	BAB23927 BAB27511 BAB28287 BAF83416 BAI46567
EMBL	CAC24710
GB	AAD27733 AAD40376 AAH15802 AAH62136 AAI02911
REF	NP_001029898 NP_001253784 NP_001296170 NP_057036 NP_079890
SP	Q99ND9 Q9CQK7 Q9H446
TPG	DAA26307
AlphaFold	Q99ND9 Q9CQK7 Q9H446