BMRB Entry 11335

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PDB ID: 2ea5
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11335
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the RING domain of the human Cell growth regulator with RING finger domain 1 protein

Deposition date:

2010-08-10

Original release date:

2011-08-19

Authors:

Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the RING domain of the human Cell growth regulator with RING finger domain 1 protein" .

Assembly members:

Assembly members:
RING domain, polymer, 68 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P051017-24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RING domain: GSSGSSGVEPSEENSKDCVV CQNGTVNWVLLPCRHTCLCD GCVKYFQQCPMCRQFVQESF ALSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	245
15N chemical shifts	63
1H chemical shifts	398

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RING domain	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, RING domain 68 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

VAL

GLU

PRO

2

SER

GLU

ASN

SER

LYS

ASP

CYS

VAL

3

CYS

GLN

ASN

GLY

THR

VAL

ASN

TRP

VAL

LEU

4

LEU

PRO

CYS

ARG

HIS

THR

CYS

LEU

CYS

ASP

5

GLY

CYS

VAL

LYS

TYR

PHE

GLN

CYS

PRO

6

MET

CYS

ARG

GLN

PHE

VAL

GLN

GLU

SER

PHE

7

ALA

LEU

SER

GLY

PRO

SER

GLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: RING domain, [U-13C; U-15N], 1.10 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1.0 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

TOPSPIN v1.3, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE II 900 MHz

PDB	2EA5
REF	XP_003987699 XP_004279384 XP_004324763 XP_005685937 XP_007062773