BMRB Entry 11314

Title:
Solution Structure of the RING domain of the Synaptotagmin-like protein 4
Deposition date:
2010-08-10
Original release date:
2011-08-19
Authors:
Miyamoto, K.; Sato, M.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Miyamoto, K.; Sato, M.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the RING domain of the Synaptotagmin-like protein 4"  .

Assembly members:

Assembly members:
RING domain, polymer, 76 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P041101-16

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts268
15N chemical shifts61
1H chemical shifts412

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RING domain1
2ZINC ION no.12
3ZINC ION no.22

Entities:

Entity 1, RING domain 76 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLEULEUGLU
2   ILELYSARGLYSGLYALALYSARGGLYSER
3   GLNHISTYRSERASPARGTHRCYSALAARG
4   CYSGLNGLUSERLEUGLYARGLEUSERPRO
5   LYSTHRASNTHRCYSARGGLYCYSASNHIS
6   LEUVALCYSARGASPCYSARGILEGLNGLU
7   SERASNGLYTHRTRPARGCYSLYSVALCYS
8   SERGLYPROSERSERGLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: RING domain, [U-13C; U-15N], 1.37 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; NTA 0.1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.925, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAC04287 BAF85277 BAG10233 BAG35911
EMBL CAD97652 CAI42004 CAI42005
GB AAH14913 AAQ14492 AIC57515 EAX02813 EAX02814
REF NP_001123368 NP_001167539 NP_542775 XP_002808587 XP_003276489
SP Q96C24
AlphaFold Q96C24

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks