BMRB Entry 11301

Title:
Solution structure of the Myb_DNA-binding domain of human Cell division cycle 5-like protein
Deposition date:
2010-08-09
Original release date:
2011-08-19
Authors:
Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the Myb_DNA-binding domain of human Cell division cycle 5-like protein"  .

Assembly members:

Assembly members:
Myb_DNA-binding domain, polymer, 66 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050905-03

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Myb_DNA-binding domain: GSSGSSGKKTEWSREEEEKL LHLAKLMPTQWRTIAPIIGR TAAQCLEHYEFLLDKAAQRD SGPSSG

Data sets:
Data typeCount
13C chemical shifts249
15N chemical shifts56
1H chemical shifts399

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Myb_DNA-binding domain1

Entities:

Entity 1, Myb_DNA-binding domain 66 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLYSLYSTHR
2   GLUTRPSERARGGLUGLUGLUGLULYSLEU
3   LEUHISLEUALALYSLEUMETPROTHRGLN
4   TRPARGTHRILEALAPROILEILEGLYARG
5   THRALAALAGLNCYSLEUGLUHISTYRGLU
6   PHELEULEUASPLYSALAALAGLNARGASP
7   SERGLYPROSERSERGLY

Samples:

sample_1: Myb_DNA-binding domain, [U-13C; U-15N], 1.13 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9736, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks