BMRB Entry 11290

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PDB ID: 1x5x
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11290
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the fibronectin type-III domain of human fibronectin type III domain containing protein 3

Deposition date:

2010-08-09

Original release date:

2011-08-18

Authors:

Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the fibronectin type-III domain of human fibronectin type III domain containing protein 3" .

Assembly members:

Assembly members:
Fibronectin type-III domain, polymer, 109 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040802-05

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Fibronectin type-III domain: GSSGSSGPSMPASPVLTKAG ITWLSLQWSKPSGTPSDEGI SYILEMEEETSGYGFKPKYD GEDLAYTVKNLRRSTKYKFK VIAYNSEGKSNPSEVVEFTT CPDSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	437
15N chemical shifts	93
1H chemical shifts	668

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Fibronectin type-III domain	1

Entities:

Entity 1, Fibronectin type-III domain 109 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PRO

SER

MET

2

PRO

ALA

SER

PRO

VAL

LEU

THR

LYS

ALA

GLY

3

ILE

THR

TRP

LEU

SER

LEU

GLN

TRP

SER

LYS

4

PRO

SER

GLY

THR

PRO

SER

ASP

GLU

GLY

ILE

5

SER

TYR

ILE

LEU

GLU

MET

GLU

THR

6

SER

GLY

TYR

GLY

PHE

LYS

PRO

LYS

TYR

ASP

7

GLY

GLU

ASP

LEU

ALA

TYR

THR

VAL

LYS

ASN

8

LEU

ARG

SER

THR

LYS

TYR

LYS

PHE

LYS

9

VAL

ILE

ALA

TYR

ASN

SER

GLU

GLY

LYS

SER

10

ASN

PRO

SER

GLU

VAL

GLU

PHE

THR

11

CYS

PRO

ASP

SER

GLY

PRO

SER

GLY

Samples:

sample_1: fn3 domain, [U-13C; U-15N], 1.06 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v1.0.8, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz