BMRB Entry 11285

Name: Solution structure of the SH3 domain of mouse peroxisomal biogenesis factor 13
Published: 2011-08-18

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PDB ID: 1wxu
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11285
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the SH3 domain of mouse peroxisomal biogenesis factor 13

Deposition date:

2010-08-09

Original release date:

2011-08-18

Authors:

Yoneyama, M.; Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Yoneyama, M.; Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the SH3 domain of mouse peroxisomal biogenesis factor 13" .

Assembly members:

Assembly members:
SH3 domain, polymer, 93 residues, Formula weight is not available

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040802-03

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SH3 domain: GSSGSSGTNWASGEDDHVVA RAEYDFVAVSDEEISFRAGD MLNLALKEQQPKVRGWLLAS LDGQTTGLIPANYVKILGKR RGRKTIESGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	343
15N chemical shifts	86
1H chemical shifts	586

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SH3 domain	1

Entities:

Entity 1, SH3 domain 93 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

THR

ASN

TRP

2

ALA

SER

GLY

GLU

ASP

HIS

VAL

ALA

3

ARG

ALA

GLU

TYR

ASP

PHE

VAL

ALA

VAL

SER

4

ASP

GLU

ILE

SER

PHE

ARG

ALA

GLY

ASP

5

MET

LEU

ASN

LEU

ALA

LEU

LYS

GLU

GLN

6

PRO

LYS

VAL

ARG

GLY

TRP

LEU

ALA

SER

7

LEU

ASP

GLY

GLN

THR

GLY

LEU

ILE

PRO

8

ALA

ASN

TYR

VAL

LYS

ILE

LEU

GLY

LYS

ARG

9

ARG

GLY

ARG

LYS

THR

ILE

GLU

SER

GLY

PRO

10

SER

GLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1.09 mM; d-Tris-HCl 20 mM; NaCl 200 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 220 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
4D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.921, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 700 MHz

PDB	1WXU
DBJ	BAA88906
GB	EGW11607 ELW63346 ERE88484
REF	NP_001233734 XP_008842699