BMRB Entry 11264

Name: Solution Structure of Ras-binding Domain in Mouse AF-6 Protein
Published: 2011-08-18

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PDB ID: 1wxa
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11264
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of Ras-binding Domain in Mouse AF-6 Protein

Deposition date:

2010-08-09

Original release date:

2011-08-18

Authors:

Zhao, C.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Zhao, C.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of Ras-binding Domain in Mouse AF-6 Protein" .

Assembly members:

Assembly members:
Ras-binding domain, polymer, 116 residues, Formula weight is not available

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040719-08

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ras-binding domain: GSSGSSGSGGTLRIYADSLK PNIPYKTILLSTTDTADFAV AESLEKYGLEKENPKDYCIA RVMLPPGAQHSDERGAKEII LDDDECPLQIFREWPSDKGI LVFQLKRRPPSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	470
15N chemical shifts	100
1H chemical shifts	742

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ras-binding domain	1

Entities:

Entity 1, Ras-binding domain 116 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

SER

GLY

2

THR

LEU

ARG

ILE

TYR

ALA

ASP

SER

LEU

LYS

3

PRO

ASN

ILE

PRO

TYR

LYS

THR

ILE

LEU

4

SER

THR

ASP

THR

ALA

ASP

PHE

ALA

VAL

5

ALA

GLU

SER

LEU

GLU

LYS

TYR

GLY

LEU

GLU

6

LYS

GLU

ASN

PRO

LYS

ASP

TYR

CYS

ILE

ALA

7

ARG

VAL

MET

LEU

PRO

GLY

ALA

GLN

HIS

8

SER

ASP

GLU

ARG

GLY

ALA

LYS

GLU

ILE

9

LEU

ASP

GLU

CYS

PRO

LEU

GLN

ILE

10

PHE

ARG

GLU

TRP

PRO

SER

ASP

LYS

GLY

ILE

11

LEU

VAL

PHE

GLN

LEU

LYS

ARG

PRO

12

SER

GLY

PRO

SER

GLY

Samples:

sample_1: Ras-binding domain, [U-13C; U-15N], 1.28 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298.0 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.921, Kobayashi, N. - data analysis

CYANA v1.0.8, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1WXA
GB	AAH14505