BMRB Entry 11252

Name: Resonance assignments of HRDC domain from Bloom syndrome protein
Published: 2010-09-28

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PDB ID: 2rrd
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11252
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Resonance assignments of HRDC domain from Bloom syndrome protein

Deposition date:

2010-08-05

Original release date:

2010-09-28

Authors:

Sato, Akiko; Mishima, Masaki; Nagai, Aki; Kim, Sun-Yong; Ito, Yutaka; Hakoshima, Toshio; Jee, Jun-Goo; Kitano, Ken

Citation:

Citation: Sato, Akiko; Mishima, Masaki; Nagai, Aki; Kim, Sun-Yong; Ito, Yutaka; Hakoshima, Toshio; Jee, Jun-Goo; Kitano, Ken. "Resonance assignments of HRDC domain from Bloom syndrome protein" J. Biochem. ., .-..

Assembly members:

Assembly members:
BLM HRDC domain, polymer, 101 residues, 11282.033 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-5X-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
BLM HRDC domain: GIPEFKQKALVAKVSQREEM VKKCLGELTEVCKSLGKVFG VHYFNIFNTVTLKKLAESLS SDPEVLLQIDGVTEDKLEKY GAEVISVLQKYSEWTSPAED S

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	295
15N chemical shifts	88
1H chemical shifts	590

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	BLM HRDC domain	1

Entities:

Entity 1, BLM HRDC domain 101 residues - 11282.033 Da.

Residues 1-5 represent a non-native expression tag

1

GLY

ILE

PRO

GLU

PHE

LYS

GLN

LYS

ALA

LEU

2

VAL

ALA

LYS

VAL

SER

GLN

ARG

GLU

MET

3

VAL

LYS

CYS

LEU

GLY

GLU

LEU

THR

GLU

4

VAL

CYS

LYS

SER

LEU

GLY

LYS

VAL

PHE

GLY

5

VAL

HIS

TYR

PHE

ASN

ILE

PHE

ASN

THR

VAL

6

THR

LEU

LYS

LEU

ALA

GLU

SER

LEU

SER

7

SER

ASP

PRO

GLU

VAL

LEU

GLN

ILE

ASP

8

GLY

VAL

THR

GLU

ASP

LYS

LEU

GLU

LYS

TYR

9

GLY

ALA

GLU

VAL

ILE

SER

VAL

LEU

GLN

LYS

10

TYR

SER

GLU

TRP

THR

SER

PRO

ALA

GLU

ASP

11

SER

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
4D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HNHB	sample_2	isotropic	sample_conditions_1
3D HN(CO)HB	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_3	isotropic	sample_conditions_1

BMRB	16766
PDB	2KV2 2RRD 4CDG 4CGZ 4O3M
DBJ	BAG36927 BAH12008 BAH13907
GB	AAA87850 AAH93622 AAI01568 AAI15031 AAI15033
REF	NP_000048 NP_001274175 NP_001274177 XP_001097543 XP_002749167
SP	P54132
AlphaFold	P54132

BMRB Entry 11252

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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