BMRB Entry 11249
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11249
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Citation: Wang, Lei; Ren, Xiaobai; Li, Yifei; Rouhier, Nicolas; Jacquot, Jean-Pierre; Jin, Changwen; Xia, Bin. "1H, 13C, and 15N resonance assignments of reduced GrxS14 from Populus tremula tremuloides." Biomol. NMR Assignments 5, 121-124 (2011).
PubMed: 21086077
Assembly members:
apo GrxS14, polymer, 109 residues, 12184.04 Da.
GSH, non-polymer, 307.323 Da.
FES, non-polymer, 175.820 Da.
Natural source: Common Name: black cottonwood Taxonomy ID: 3694 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Populus trichocarpa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3d
Entity Sequences (FASTA):
apo GrxS14: MALTPALKTTLDKVVTSHKV
VLFMKGTKDFPQCGFSQTVV
QILKSLNAPFESVNILENEL
LRQGLKEYSSWPTFPQLYID
GEFFGGCDITVEAYKSGELQ
EQVEKAMCS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 409 |
| 15N chemical shifts | 97 |
| 1H chemical shifts | 690 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | apo GrxS14 no.1 | 1 |
| 2 | apo GrxS14 no.2 | 1 |
| 3 | glutathione no.1 | 2 |
| 4 | glutathione no.2 | 2 |
| 5 | [2Fe-2S] cluster | 3 |
Entities:
Entity 1, apo GrxS14 no.1 109 residues - 12184.04 Da.
| 1 | MET | ALA | LEU | THR | PRO | ALA | LEU | LYS | THR | THR | ||||
| 2 | LEU | ASP | LYS | VAL | VAL | THR | SER | HIS | LYS | VAL | ||||
| 3 | VAL | LEU | PHE | MET | LYS | GLY | THR | LYS | ASP | PHE | ||||
| 4 | PRO | GLN | CYS | GLY | PHE | SER | GLN | THR | VAL | VAL | ||||
| 5 | GLN | ILE | LEU | LYS | SER | LEU | ASN | ALA | PRO | PHE | ||||
| 6 | GLU | SER | VAL | ASN | ILE | LEU | GLU | ASN | GLU | LEU | ||||
| 7 | LEU | ARG | GLN | GLY | LEU | LYS | GLU | TYR | SER | SER | ||||
| 8 | TRP | PRO | THR | PHE | PRO | GLN | LEU | TYR | ILE | ASP | ||||
| 9 | GLY | GLU | PHE | PHE | GLY | GLY | CYS | ASP | ILE | THR | ||||
| 10 | VAL | GLU | ALA | TYR | LYS | SER | GLY | GLU | LEU | GLN | ||||
| 11 | GLU | GLN | VAL | GLU | LYS | ALA | MET | CYS | SER |
Entity 2, glutathione no.1 - C10 H17 N3 O6 S - 307.323 Da.
| 1 | GSH |
Entity 3, [2Fe-2S] cluster - Fe2 S2 - 175.820 Da.
| 1 | FES |
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