BMRB Entry 11236

Title:
Solution structure of the fourth Ig-like domain from myosin light chain kinase, smooth muscle
Deposition date:
2010-07-23
Original release date:
2011-08-03
Authors:
Qin, X.; Kurosaki, C.; Yoshida, M.; Hayahsi, F.; Yokoyama, S.
Citation:

Citation: Qin, X.; Kurosaki, C.; Yoshida, M.; Hayashi, F.; Yokoyama, S.. "Solution structure of the fourth Ig-like domain from myosin light chain kinase, smooth muscle"  .

Assembly members:

Assembly members:
Ig domain, polymer, 99 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P061030-13

Data sets:
Data typeCount
13C chemical shifts400
15N chemical shifts96
1H chemical shifts637

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ig domain1

Entities:

Entity 1, Ig domain 99 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYMETGLUVAL
2   ALAPROSERPHESERSERVALLEULYSASP
3   CYSALAVALILEGLUGLYGLNASPPHEVAL
4   LEUGLNCYSSERVALARGGLYTHRPROVAL
5   PROARGILETHRTRPLEULEUASNGLYGLN
6   PROILEGLNTYRALAARGSERTHRCYSGLU
7   ALAGLYVALALAGLULEUHISILEGLNASP
8   ALALEUPROGLUASPHISGLYTHRTYRTHR
9   CYSLEUALAGLUASNALALEUGLYGLNVAL
10   SERCYSSERALATRPVALTHRVALHIS

Samples:

sample_1: Ig domain, [U-13C; U-15N], 1.13 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

Delta v4.3, JEOL - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • JEOL ECA 700 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks