BMRB Entry 11175

Name: Solution structure of calponin homology domain of IQGAP1
Published: 2010-09-09

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PDB ID: 2rr8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11175
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of calponin homology domain of IQGAP1

Deposition date:

2010-06-09

Original release date:

2010-09-09

Authors:

Umemoto, Ryo; Nishida, Noritaka; Ogino, Shinji; Shimada, Ichio

Citation:

Citation: Umemoto, Ryo; Nishida, Noritaka; Ogino, Shinji; Shimada, Ichio. "NMR structure of the calponin homology domain of human IQGAP1 and its implications for the actin recognition mode" J. Biomol. NMR 48, 59-64 (2010).
PubMed: 20644981

Assembly members:

Assembly members:
CH domain, polymer, 190 residues, 22032.408 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6p

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CH domain: GPLGSLTAEEMDERRRQNVA YEYLCHLEEAKRWMEACLGE DLPPTTELEEGLRNGVYLAK LGNFFSPKVVSLKKIYDREQ TRYKATGLHFRHTDNVIQWL NAMDEIGLPKIFYPETTDIY DRKNMPRCIYCIHALSLYLF KLGLAPQIQDLYGKVDFTEE EINNMKTELEKYGIQMPAFS KIGGILANEL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	651
15N chemical shifts	180
1H chemical shifts	1262

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CH domain	1

Entities:

Entity 1, CH domain 190 residues - 22032.408 Da.

1	GLY	PRO	LEU	GLY	SER	LEU	THR	ALA	GLU	GLU
2	MET	ASP	GLU	ARG	ARG	ARG	GLN	ASN	VAL	ALA
3	TYR	GLU	TYR	LEU	CYS	HIS	LEU	GLU	GLU	ALA
4	LYS	ARG	TRP	MET	GLU	ALA	CYS	LEU	GLY	GLU
5	ASP	LEU	PRO	PRO	THR	THR	GLU	LEU	GLU	GLU
6	GLY	LEU	ARG	ASN	GLY	VAL	TYR	LEU	ALA	LYS
7	LEU	GLY	ASN	PHE	PHE	SER	PRO	LYS	VAL	VAL
8	SER	LEU	LYS	LYS	ILE	TYR	ASP	ARG	GLU	GLN
9	THR	ARG	TYR	LYS	ALA	THR	GLY	LEU	HIS	PHE
10	ARG	HIS	THR	ASP	ASN	VAL	ILE	GLN	TRP	LEU
11	ASN	ALA	MET	ASP	GLU	ILE	GLY	LEU	PRO	LYS
12	ILE	PHE	TYR	PRO	GLU	THR	THR	ASP	ILE	TYR
13	ASP	ARG	LYS	ASN	MET	PRO	ARG	CYS	ILE	TYR
14	CYS	ILE	HIS	ALA	LEU	SER	LEU	TYR	LEU	PHE
15	LYS	LEU	GLY	LEU	ALA	PRO	GLN	ILE	GLN	ASP
16	LEU	TYR	GLY	LYS	VAL	ASP	PHE	THR	GLU	GLU
17	GLU	ILE	ASN	ASN	MET	LYS	THR	GLU	LEU	GLU
18	LYS	TYR	GLY	ILE	GLN	MET	PRO	ALA	PHE	SER
19	LYS	ILE	GLY	GLY	ILE	LEU	ALA	ASN	GLU	LEU

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D HCCH-COSY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_3	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

PDB	2RR8 3I6X
DBJ	BAA06123 BAB30486 BAC26450 BAC97854 BAE37174
EMBL	CAH89415
GB	AAA59187 AAF60344 AAH05906 AAH20549 AAH22105
REF	NP_001101959 NP_003861 NP_057930 XP_001364413 XP_002804991
SP	P46940 Q9JKF1
TPG	DAA17679
AlphaFold	P46940 Q9JKF1

BMRB Entry 11175

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	GLY	PRO	LEU	GLY	SER	LEU	THR	ALA	GLU	GLU
2	MET	ASP	GLU	ARG	ARG	ARG	GLN	ASN	VAL	ALA
3	TYR	GLU	TYR	LEU	CYS	HIS	LEU	GLU	GLU	ALA
4	LYS	ARG	TRP	MET	GLU	ALA	CYS	LEU	GLY	GLU
5	ASP	LEU	PRO	PRO	THR	THR	GLU	LEU	GLU	GLU
6	GLY	LEU	ARG	ASN	GLY	VAL	TYR	LEU	ALA	LYS
7	LEU	GLY	ASN	PHE	PHE	SER	PRO	LYS	VAL	VAL
8	SER	LEU	LYS	LYS	ILE	TYR	ASP	ARG	GLU	GLN
9	THR	ARG	TYR	LYS	ALA	THR	GLY	LEU	HIS	PHE
10	ARG	HIS	THR	ASP	ASN	VAL	ILE	GLN	TRP	LEU
11	ASN	ALA	MET	ASP	GLU	ILE	GLY	LEU	PRO	LYS
12	ILE	PHE	TYR	PRO	GLU	THR	THR	ASP	ILE	TYR
13	ASP	ARG	LYS	ASN	MET	PRO	ARG	CYS	ILE	TYR
14	CYS	ILE	HIS	ALA	LEU	SER	LEU	TYR	LEU	PHE
15	LYS	LEU	GLY	LEU	ALA	PRO	GLN	ILE	GLN	ASP
16	LEU	TYR	GLY	LYS	VAL	ASP	PHE	THR	GLU	GLU
17	GLU	ILE	ASN	ASN	MET	LYS	THR	GLU	LEU	GLU
18	LYS	TYR	GLY	ILE	GLN	MET	PRO	ALA	PHE	SER
19	LYS	ILE	GLY	GLY	ILE	LEU	ALA	ASN	GLU	LEU

1	GLY	PRO	LEU	GLY	SER	LEU	THR	ALA	GLU	GLU
2	MET	ASP	GLU	ARG	ARG	ARG	GLN	ASN	VAL	ALA
3	TYR	GLU	TYR	LEU	CYS	HIS	LEU	GLU	GLU	ALA
4	LYS	ARG	TRP	MET	GLU	ALA	CYS	LEU	GLY	GLU
5	ASP	LEU	PRO	PRO	THR	THR	GLU	LEU	GLU	GLU
6	GLY	LEU	ARG	ASN	GLY	VAL	TYR	LEU	ALA	LYS
7	LEU	GLY	ASN	PHE	PHE	SER	PRO	LYS	VAL	VAL
8	SER	LEU	LYS	LYS	ILE	TYR	ASP	ARG	GLU	GLN
9	THR	ARG	TYR	LYS	ALA	THR	GLY	LEU	HIS	PHE
10	ARG	HIS	THR	ASP	ASN	VAL	ILE	GLN	TRP	LEU
11	ASN	ALA	MET	ASP	GLU	ILE	GLY	LEU	PRO	LYS
12	ILE	PHE	TYR	PRO	GLU	THR	THR	ASP	ILE	TYR
13	ASP	ARG	LYS	ASN	MET	PRO	ARG	CYS	ILE	TYR
14	CYS	ILE	HIS	ALA	LEU	SER	LEU	TYR	LEU	PHE
15	LYS	LEU	GLY	LEU	ALA	PRO	GLN	ILE	GLN	ASP
16	LEU	TYR	GLY	LYS	VAL	ASP	PHE	THR	GLU	GLU
17	GLU	ILE	ASN	ASN	MET	LYS	THR	GLU	LEU	GLU
18	LYS	TYR	GLY	ILE	GLN	MET	PRO	ALA	PHE	SER
19	LYS	ILE	GLY	GLY	ILE	LEU	ALA	ASN	GLU	LEU

1	GLY	PRO	LEU	GLY	SER	LEU	THR	ALA	GLU	GLU
2	MET	ASP	GLU	ARG	ARG	ARG	GLN	ASN	VAL	ALA
3	TYR	GLU	TYR	LEU	CYS	HIS	LEU	GLU	GLU	ALA
4	LYS	ARG	TRP	MET	GLU	ALA	CYS	LEU	GLY	GLU
5	ASP	LEU	PRO	PRO	THR	THR	GLU	LEU	GLU	GLU
6	GLY	LEU	ARG	ASN	GLY	VAL	TYR	LEU	ALA	LYS
7	LEU	GLY	ASN	PHE	PHE	SER	PRO	LYS	VAL	VAL
8	SER	LEU	LYS	LYS	ILE	TYR	ASP	ARG	GLU	GLN
9	THR	ARG	TYR	LYS	ALA	THR	GLY	LEU	HIS	PHE
10	ARG	HIS	THR	ASP	ASN	VAL	ILE	GLN	TRP	LEU
11	ASN	ALA	MET	ASP	GLU	ILE	GLY	LEU	PRO	LYS
12	ILE	PHE	TYR	PRO	GLU	THR	THR	ASP	ILE	TYR
13	ASP	ARG	LYS	ASN	MET	PRO	ARG	CYS	ILE	TYR
14	CYS	ILE	HIS	ALA	LEU	SER	LEU	TYR	LEU	PHE
15	LYS	LEU	GLY	LEU	ALA	PRO	GLN	ILE	GLN	ASP
16	LEU	TYR	GLY	LYS	VAL	ASP	PHE	THR	GLU	GLU
17	GLU	ILE	ASN	ASN	MET	LYS	THR	GLU	LEU	GLU
18	LYS	TYR	GLY	ILE	GLN	MET	PRO	ALA	PHE	SER
19	LYS	ILE	GLY	GLY	ILE	LEU	ALA	ASN	GLU	LEU