BMRB Entry 11150
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11150
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Abe, H.; Sasagawa, A.; Tochio, N.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the Somatomedin B domain of human Ectonucleotide
pyrophosphatase/phosphodiesterase family member" .
Assembly members:
somatomedin B, polymer, 56 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050118-03
Entity Sequences (FASTA):
somatomedin B: GSSGSSGWTCNKFRCGEKRL
TRSLCACSDDCKDQGDCCIN
YSSVCQGEKSSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 172 |
| 15N chemical shifts | 47 |
| 1H chemical shifts | 263 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | somatomedin B | 1 |
Entities:
Entity 1, somatomedin B 56 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | TRP | THR | CYS | ||||
| 2 | ASN | LYS | PHE | ARG | CYS | GLY | GLU | LYS | ARG | LEU | ||||
| 3 | THR | ARG | SER | LEU | CYS | ALA | CYS | SER | ASP | ASP | ||||
| 4 | CYS | LYS | ASP | GLN | GLY | ASP | CYS | CYS | ILE | ASN | ||||
| 5 | TYR | SER | SER | VAL | CYS | GLN | GLY | GLU | LYS | SER | ||||
| 6 | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: somatomedin B, [U-13C; U-15N], 0.80 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9820, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - structure solution
NMR spectrometers:
- Bruker AVANCE 700 MHz
Related Database Links:
| PDB | |
| DBJ | BAA02054 BAG37385 |
| EMBL | CAC39442 |
| GB | AAA63237 AAD38420 AAD38421 AAF36094 AAH59375 |
| REF | NP_001162404 NP_006199 XP_001103359 XP_002747012 XP_002817408 |
| SP | P22413 |
| AlphaFold | P22413 |
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